Document 0265
 DOCN  M9550265
 TI    Detection of human T-lymphotropic virus type I (HTLV-I) tax RNA in the
       central nervous system of HTLV-I-associated myelopathy/tropical spastic
       paraparesis patients by in situ hybridization [see comments]
 DT    9505
 AU    Lehky TJ; Fox CH; Koenig S; Levin MC; Flerlage N; Izumo S; Sato E; Raine
       CS; Osame M; Jacobson S; Neuroimmunology Branch, National Institute of
       Neurological; Diseases and Stroke, National Institutes of Health,
       Bethesda, MD; 20892.
 SO    Ann Neurol. 1995 Feb;37(2):167-75. Unique Identifier : AIDSLINE
       MED/95150535
 CM    Comment in: Ann Neurol 1995 Feb;37(2):143-5
 AB    Autopsy specimens from 3 patients with human T-lymphotropic virus
       (HTLV-I)-associated myelopathy/tropical spastic paraparesis (HAM/TSP)
       were examined for the presence of HTLV-I in the central nervous system
       (CNS). In situ hybridization using an HTLV-I tax RNA probe detected
       cells containing HTLV-I RNA in spinal cord and cerebellar sections.
       HTLV-I infected cells were located within the white matter and, in
       particular, within the anterior and lateral funiculi of the spinal cord.
       Consistent with previously described HAM/TSP pathology, there were
       perivascular infiltrates in these CNS specimens. Significantly, HTLV-I
       RNA was not localized to these infiltrates but was detected deeper
       within the neural tissue. Furthermore, phenotypic analysis demonstrated
       that at least some of the infected cells were astrocytes. While previous
       polymerase chain reaction studies have demonstrated the presence of
       proviral HTLV-I in CNS specimens, here we provide evidence for the in
       situ expression of HTLV-I RNA in the CNS of HAM/TSP patients.
 DE    Aged  Cerebellum/*VIROLOGY  Female  Gene Products, tax/*ANALYSIS  Human
       HTLV-I/*ISOLATION & PURIF  In Situ Hybridization  Male  Paraparesis,
       Tropical Spastic/*VIROLOGY  Polymerase Chain Reaction  RNA,
       Viral/*ANALYSIS  Spinal Cord/*VIROLOGY  Support, Non-U.S. Gov't  JOURNAL
       ARTICLE

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