Document 0278
 DOCN  M9550278
 TI    Adult T-cell leukemia/lymphoma with two distinct clones in the
       peripheral blood and lymph node.
 DT    9505
 AU    Shibata K; Shimamoto Y; Suga K; Watanabe M; Kikuchi M; Yamaguchi M;
       Department of Internal Medicine, Saga Medical School, Japan.
 SO    Am J Hematol. 1995 Feb;48(2):116-9. Unique Identifier : AIDSLINE
       MED/95149976
 AB    A case of adult T-cell leukemia/lymphoma (ATL) with two different clones
       in the peripheral blood and lymph nodes is reported here. When cellular
       DNA from the lymph node was digested with EcoRI, one band larger than 9
       Kb was detected. Digestion of the cellular DNA with PstI resulted in one
       clear band in addition to three internal fragments. In contrast, when
       cellular DNA from malignant peripheral blood lymphocytes (PBL) was
       digested with the same endonucleases, distinct bands at positions
       different from those observed in the lymph node were detected,
       indicating two separate malignant clones in the patient. Monoclonality
       of the tumor cells was shown by T-cell receptor-beta (TCR-beta) gene
       rearrangement in both PBL and lymph node. Furthermore, there was a
       difference in the surface phenotype between tumor cells taken from
       peripheral blood (CD4+, CD8-) and lymph node (CD4+, CD8+). These
       findings suggest the presence of two different ATL clones in PBL and
       lymph node in a single patient simultaneously, which is distinguishable
       by the integration pattern of human T-cell leukemia virus type I
       (HTLV-I) proviral DNA.
 DE    Aged  Aged, 80 and over  Blotting, Southern  Case Report  Clone
       Cells/*PATHOLOGY  Deoxyribonuclease EcoRI  DNA, Viral/ANALYSIS  Gene
       Rearrangement, beta-Chain T-Cell Antigen Receptor  Human
       HTLV-I/GENETICS  HTLV-I Infections/*PATHOLOGY/VIROLOGY  Leukemia,
       T-Cell/*PATHOLOGY/VIROLOGY  Lymph Nodes/*PATHOLOGY
       Lymphoma/*PATHOLOGY/VIROLOGY  Male  Support, Non-U.S. Gov't
       T-Lymphocyte Subsets  JOURNAL ARTICLE

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