Document 0287
 DOCN  M9550287
 TI    Diagnosis of progressive multifocal leukoencephalopathy by stereotactic
       brain biopsy utilizing immunohistochemistry and the polymerase chain
       reaction.
 DT    9505
 AU    Silver SA; Arthur RR; Erozan YS; Sherman ME; McArthur JC; Uematsu S;
       Department of Pathology, Johns Hopkins Medical Institutions,; Baltimore,
       Maryland.
 SO    Acta Cytol. 1995 Jan-Feb;39(1):35-44. Unique Identifier : AIDSLINE
       MED/95149621
 AB    This report describes the diagnosis of progressive multifocal
       leukoencephalopathy (PML) in nine patients using cytopathologic and
       histopathologic examination of computed tomographically guided
       stereotactic brain biopsies in combination with immunostaining for
       SV-40-related antigen and the polymerase chain reaction (PCR) for the JC
       virus. In four patients the diagnosis of PML was based on the
       microscopic appearance of the biopsies and immunostaining for
       SV-40-related antigen. In one of these patients the diagnosis was also
       supported by PCR for the JC virus. In two patients whose biopsies were
       only suggestive of PML, a definitive diagnosis was possible utilizing
       immunohistochemistry and PCR. In another case the histopathologic
       features were atypical of PML, and the diagnosis was established with
       immunostaining and PCR. The diagnosis of PML was established by PCR
       alone in two patients whose biopsies showed only suggestive or
       nonspecific findings. We conclude that the accuracy of stereotactic
       biopsy in the diagnosis of PML is enhanced by using a combination of
       light microscopy, immunohistochemistry and PCR.
 DE    Adult  Antigens, Polyomavirus Transforming/ANALYSIS  Base Sequence
       Biopsy/METHODS  Brain/*PATHOLOGY/RADIOGRAPHY  DNA,
       Viral/ANALYSIS/GENETICS  Genome, Viral  Human  HIV
       Infections/COMPLICATIONS  Immunohistochemistry  Leukoencephalopathy,
       Progressive Multifocal/COMPLICATIONS/  *DIAGNOSIS/*PATHOLOGY  Magnetic
       Resonance Imaging  Male  Middle Age  Molecular Sequence Data
       Oligonucleotide Probes/ANALYSIS/CHEMISTRY/GENETICS  Polymerase Chain
       Reaction  Polyomavirus hominis 2/GENETICS  Support, U.S. Gov't, P.H.S.
       Tomography, X-Ray Computed  JOURNAL ARTICLE

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