Document 0357
 DOCN  M9550357
 TI    Human immunodeficiency virus type 2 (HIV-2) env gene analysis:
       prediction of glycoprotein epitopes important for heterotypic
       neutralization and evidence for three genotype clusters within the
       HIV-2a subtype.
 DT    9505
 AU    Breuer J; Douglas NW; Goldman N; Daniels RS; National Institute for
       Medical Research, The Ridgeway, Mill Hill,; London, UK.
 SO    J Gen Virol. 1995 Feb;76 ( Pt 2):333-45. Unique Identifier : AIDSLINE
       MED/95146974
 AB    The env gene sequences of ten tissue-culture-adapted human
       immunodeficiency virus type 2 (HIV-2) isolates from West African
       patients were determined. Alignment and comparison of the gene sequences
       and putative translation products with database sequences revealed
       11-29% diversity at the nucleotide level and 15-31% variation at the
       protein level. From analysis of glycoproteins of HIV-2 strains sensitive
       and resistant to neutralization by HIV-1 antisera, five regions were
       identified as putative targets for cross-neutralizing antibody. The
       HIV-2 equivalent of the HIV-1 V3 loop was not included in this number.
       However, three of the HIV-2 peptides aligned with regions identified as
       targets for broad neutralization of HIV-1 strains. These were the V2 and
       CD4-binding domains of gp120 and the Kennedy domain in gp41.
       Phylogenetic analysis of the env gene sequences, together with HIV-2 env
       gene sequences published in the Los Alamos database, support the
       identification of two distinct HIV-2 subtypes, HIV-2a and HIV-2b. The
       new sequences are located within the HIV-2a subtype and allow prediction
       of at least three genotypes, designated I-III. Some correlation of
       genotype with geographical origin of isolates was noted. Genotype I
       viruses originate from Guinea Bissau and group II viruses mainly
       originate from The Gambia. One isolate from Guinea Bissau, HIV-2CAM4,
       appears phylogenetically older than other viruses in the HIV-2a subtype.
       The possible implications of this in the light of epidemiological
       findings in Guinea Bissau are discussed.
 DE    Amino Acid Sequence  *Antigenic Determinants  Base Sequence  *Genes, env
       Genotype  Human  HIV Envelope Protein gp120/*IMMUNOLOGY  HIV Envelope
       Protein gp41/*IMMUNOLOGY  HIV-2/CLASSIFICATION/*GENETICS/IMMUNOLOGY
       Immune Sera/IMMUNOLOGY  Molecular Sequence Data  Neutralization Tests
       Support, Non-U.S. Gov't  SIV/GENETICS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).