Document 0358
 DOCN  M9550358
 TI    Sequence of host-range determinants in the env gene of a full-length,
       infectious proviral clone of exogenous avian leukosis virus HPRS-103
       confirms that it represents a new subgroup (designated J).
 DT    9505
 AU    Bai J; Howes K; Payne LN; Skinner MA; Department of Molecular Biology,
       Institute for Animal Health,; Compton, Nr Newbury, Berks, UK.
 SO    J Gen Virol. 1995 Jan;76 ( Pt 1):181-7. Unique Identifier : AIDSLINE
       GENBANK/Z36973
 AB    A genomic DNA library was constructed, in a bacteriophage lambda vector,
       from line 0 chick embryo fibroblasts (CEFs) infected with HPRS-103, an
       exogenous avian leukosis virus (ALV; envelope subgroup J) recently
       isolated from meat-type chickens. The library was screened at high
       stringency using a full length RAV-1 (subgroup A) proviral probe. From
       10(6) plaques, two clones which hybridized strongly to the RAV-1 probe
       were isolated; one contained a full-length copy of the proviral genome
       of HPRS-103 and the other contained a copy lacking the 5'-long terminal
       repeat (LTR) and part of gag. The relative strength of hybridization of
       RAV-1 and HPRS-103 clones, to RAV-1 probes representing different parts
       of the proviral genome, indicated that the gag and pol genes of HPRS-103
       share a high level of identity with those of RAV-1 but that the env gene
       and the LTRs are considerably less well conserved. Infectious virus was
       recovered from CEFs transfected with the full-length clone, as detected
       by ELISA. The recovered virus appeared to be identical to HPRS-103 by
       electron microscopy and by Southern blotting of proviral DNA. The
       recovered virus was shown to be of the same subgroup as HPRS-103 by
       serum neutralization and receptor interference assays. Sequence analysis
       of the env gene of HPRS-103 shows that it differs considerably from the
       env genes of other ALV subgroups, particularly in the host range
       determinants, consistent with the finding that HPRS-103 represents a new
       subgroup (designated J).
 DE    Amino Acid Sequence  Animal  Chick Embryo  *Genes, env  Leukosis Virus,
       Avian/CLASSIFICATION/*GENETICS  Molecular Sequence Data
       Proviruses/*GENETICS  Repetitive Sequences, Nucleic Acid  Viral Envelope
       Proteins/CHEMISTRY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).