Document 0360
 DOCN  M9550360
 TI    Cell-mediated transmission of human T cell lymphotropic virus type I to
       human malignant trophoblastic cells results in long-term persistent
       infection.
 DT    9505
 AU    Liu X; Zachar V; Norskov-Lauritsen N; Aboagye-Mathiesen G; Zdravkovic M;
       Mosborg-Petersen P; Dalsgaard AM; Hager H; Ebbesen P; Department of
       Virus and Cancer, Danish Cancer Society, Aarhus.
 SO    J Gen Virol. 1995 Jan;76 ( Pt 1):167-73. Unique Identifier : AIDSLINE
       MED/95146946
 AB    We investigated permissiveness of the malignantly transformed
       trophoblast (choriocarcinoma) cell lines JAR, BeWo and JEG-3 to the
       human T cell lymphotropic virus type I (HTLV-I). After co-culture with
       the productively infected cell line MT-2 the choriocarcinoma cell lines
       were analysed for infection over a period of three months. The presence
       of HTLV-I viral DNA was examined by PCR using primers targeting the gag,
       pol, env and pX specific sequences. All amplified segments were found
       consistently in the cell cultures throughout the period of study.
       Further analysis that aimed to characterize the size variation of the
       integrated proviral DNA by Southern blotting revealed the presence of
       integrated proviral sequences which consisted, for the most part, of
       incomplete genomes. The presence of the full-length HTLV-I genome,
       however, was unequivocally confirmed in clonally expanded cell cultures
       derived from the originally infected parental cells. In order to analyse
       virus expression at the transcriptional level, we used reverse
       transcriptase (RT)-mediated PCR that was targeted at intra-exon regions
       (gag, pol, env and pX), and the splicing sites of the env and pX-tax/rex
       mRNAs. When compared with MT-2 cells, substantially lower levels of all
       transcripts were found in all the cell lines analysed. We were
       unsuccessful in attempts to detect viral protein expression using
       polyvalent or Tax- and Gag-specific monoclonal antibodies by Western
       blot analysis or immunoprecipitation, and we could not detect any RT
       activity released into the supernatant of the infected cells either.
       Collectively, these data suggest that the trophoblastic cells may become
       persistently but essentially non-productively infected with HTLV-I.
 DE    Base Sequence  Choriocarcinoma/*VIROLOGY  DNA, Viral/ANALYSIS  Human
       HTLV-I/GENETICS/*PHYSIOLOGY  Molecular Sequence Data  Polymerase Chain
       Reaction  Proviruses/PHYSIOLOGY  Tumor Cells, Cultured  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).