Document 0372
 DOCN  M9550372
 TI    Detection of human herpesvirus 6 in plasma of children with primary
       infection and immunosuppressed patients by polymerase chain reaction.
 DT    9505
 AU    Secchiero P; Carrigan DR; Asano Y; Benedetti L; Crowley RW; Komaroff AL;
       Gallo RC; Lusso P; Laboratory of Tumor Cell Biology, National Cancer
       Institute,; National Institutes of Health, Bethesda, Maryland 20892.
 SO    J Infect Dis. 1995 Feb;171(2):273-80. Unique Identifier : AIDSLINE
       MED/95146771
 AB    A sensitive and specific polymerase chain reaction method for the
       detection of human herpesvirus 6 (HHV-6) DNA in serum or plasma has been
       developed. In total, 157 human serum or plasma samples were studied.
       HHV-6 DNA was detected in 6 (85.7%) of 7 children with exanthem subitum,
       3 (23.1%) of 13 bone marrow transplant (BMT) recipients, 4 (22.2%) of 18
       human immunodeficiency virus (HIV)-infected patients, 1 (2.6%) of 39
       patients with chronic fatigue syndrome, and none of 37 healthy adults.
       In the HHV-6-positive BMT recipients, HHV-6 plasma DNA was transiently
       detected during episodes of fever and respiratory infection. In children
       with exanthem subitum and in 1 HIV-infected patient, the HHV-6 strains
       were characterized as variant B, whereas variant A was detected in all
       other patients. Detection of viral DNA in serum or plasma is a marker of
       active infection that can be used to investigate the role of HHV-6 in
       human disease.
 DE    Adult  Base Sequence  Bone Marrow Transplantation  Child  Child,
       Preschool  DNA, Viral/*BLOOD/GENETICS  Exanthema
       Subitum/BLOOD/COMPLICATIONS/*DIAGNOSIS  Female  Herpesviridae
       Infections/BLOOD/COMPLICATIONS/*DIAGNOSIS  Herpesvirus 6,
       Human/GENETICS/*ISOLATION & PURIF  Human  HIV Infections/COMPLICATIONS
       *Immunocompromised Host  Male  Middle Age  Molecular Sequence Data
       Polymerase Chain Reaction/*METHODS  Sensitivity and Specificity
       Support, Non-U.S. Gov't  Variation (Genetics)  Viremia/DIAGNOSIS
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).