Document 0401
 DOCN  M9550401
 TI    Effect of anti-V3 antibodies on cell-free and cell-to-cell human
       immunodeficiency virus transmission.
 DT    9505
 AU    Pantaleo G; Demarest JF; Vaccarezza M; Graziosi C; Bansal GP; Koenig S;
       Fauci AS; Laboratory of Immunoregulation, National Institute of Allergy
       and; Infectious Diseases, National Institutes of Health, Bethesda, MD;
       20892.
 SO    Eur J Immunol. 1995 Jan;25(1):226-31. Unique Identifier : AIDSLINE
       MED/95145531
 AB    The present study was undertaken to compare the effects of a
       type-specific (HIV-1 MN) anti-V3 antibody on in vitro human
       immunodeficiency virus (HIV) infection of peripheral blood mononuclear
       cells in systems of cell-free versus cell-to-cell transmission of virus.
       Anti-V3 antibody completely prevented HIV-1 infection when cell-free
       virus was the sole mechanism of infection. A significant reduction of
       the neutralizing activity of the anti-V3 antibody was observed when
       infectivity was dependent on both cell-free and cell-to-cell mechanisms
       of infection. Furthermore, when cell-to-cell transfer of virions was the
       primary mechanism of HIV-1 infection, inhibition of HIV-1 infection was
       not observed. Therefore, a potent neutralizing antibody with a single
       epitope specificity failed to effectively control dissemination of a
       persistent HIV-1 infection in a system characterized predominantly by
       cell-to-cell transfer of virus.
 DE    Amino Acid Sequence  Antibodies, Monoclonal/IMMUNOLOGY  Cell Separation
       Cell-Free System  Cells, Cultured/VIROLOGY  Flow Cytometry  Human  HIV
       Antibodies/*IMMUNOLOGY  HIV Envelope Protein gp120/*IMMUNOLOGY  HIV
       Infections/TRANSMISSION  HIV-1/*IMMUNOLOGY  Molecular Sequence Data
       Neutralization Tests  Peptide Fragments/*IMMUNOLOGY  Polymerase Chain
       Reaction  JOURNAL ARTICLE

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