Document 0406
 DOCN  M9550406
 TI    Clearance of Sendai virus by CD8+ T cells requires direct targeting to
       virus-infected epithelium.
 DT    9505
 AU    Hou S; Doherty PC; Department of Immunology, St. Jude Children's
       Research Hospital,; Memphis, TN 38105.
 SO    Eur J Immunol. 1995 Jan;25(1):111-6. Unique Identifier : AIDSLINE
       MED/95145512
 AB    Minimal numbers of CD8+ T cells are found in bronchoalveolar lavage
       (BAL) populations recovered from Sendai virus-infected mice that are
       homozygous (-/-) for a beta 2-microglobulin (beta 2-m) gene disruption.
       The prevalence of the CD8+ set was substantially increased in the
       pneumonic lungs of 8-12-week radiation chimeras made using substantially
       class I major histocompatibility complex (MHC) glycoprotein-negative
       beta 2-m (-/-) recipients and normal beta 2-m (+/+) bone marrow. Even
       so, the CD8+ (but not the CD4+) lymphocyte counts were still much lower
       than in the (+/+)-->(+/+) controls. The (+/+)-->(+/+) and (+/+)-->(-/-)
       chimeras cleared Sendai virus and potent virus-immune CD8+ cytotoxic T
       lymphocytes (CTL) specific for H-2Kb+viral nucleoprotein peptide were
       found in the BAL from both groups. However, following in vivo depletion
       of the CD4+ population, only the (+/+)-->(+/+) mice were able to deal
       with the infection. Similarly, adoptively transferred, H-2Kb-restricted
       CD8+ T cells from previously-primed (+/+) mice also failed to clear
       virus from the lungs of (+/+)-->(-/-) chimeras infected within 2 weeks
       of reconstitution with bone marrow, though they were effective in the
       (+/+)-->(+/+) controls. Sendai virus-immune CD8+ T cells are thus unable
       to eliminate virus-infected beta 2-m (-/-) lung epithelial cells that
       might be thought to be expressing very small amounts of either isolated
       class I heavy chain, or class I MHC glycoprotein that has bound beta 2-m
       derived from beta 2-m (+/+) T cells or macrophages present in the
       pneumonic lung. Furthermore, the CD8+ CTL that are being exposed to beta
       2-m (+/+) stimulators in the BAL population cannot operate in some
       bystander mode to clear virus from respiratory epithelium.
 DE    Animal  Bronchoalveolar Lavage Fluid/VIROLOGY  Cytotoxicity Tests,
       Immunologic  CD8-Positive T-Lymphocytes/*IMMUNOLOGY/TRANSPLANTATION
       Epithelium/IMMUNOLOGY/VIROLOGY  Female  Flow Cytometry  H-2
       Antigens/GENETICS  Immunotherapy, Adoptive  Mice  Mice, Inbred C57BL
       Mice, Inbred Strains  Parainfluenza/*IMMUNOLOGY/THERAPY  Parainfluenza
       Virus Type 1/*IMMUNOLOGY  Radiation Chimera/IMMUNOLOGY  Respiratory
       System/*IMMUNOLOGY/VIROLOGY  Support, Non-U.S. Gov't  Support, U.S.
       Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).