Document 0414
 DOCN  M9550414
 TI    Changes of adhesion molecule (LFA-1, ICAM-1) expression on memory T
       cells activated with cytomegalovirus antigen.
 DT    9505
 AU    Ito M; Watanabe M; Kamiya H; Sakurai M; Department of Pediatrics, Mie
       University School of Medicine,; Japan.
 SO    Cell Immunol. 1995 Jan;160(1):8-13. Unique Identifier : AIDSLINE
       MED/95144754
 AB    The expression of adhesion molecules (LFA-1, ICAM-1, CD29) on T cells
       activated with cytomegalovirus (CMV) antigen was investigated by
       three-color flow cytometry analysis. Peripheral blood mononuclear cells
       (PBMC) from CMV-seropositive adults were cultured with CMV or control
       antigen for 6 days. After culture, the expression of LFA-1, ICAM-1, and
       CD29 on T cells in subpopulations defined as CD45RO+ or CD45RO- was
       analyzed. The population of cells that expressed LFA-1 at high levels
       (LFA-1high) among CD4+C-D45RO+ cells increased when cultured with CMV
       antigen compared to control antigen. The population of cells that
       expressed LFA-1high cells among CD8+C-D45RO+ cells was down-regulated by
       culture for 6 days; however, the population of LFA-1high cells among
       CD8+CD45RO+ cells cultured with CMV antigen was higher than when
       cultured with control antigen. The population of LFA-1high cells among
       CD4+CD45RO- and CD8+CD45RO- cells did not change after culture with CMV
       antigen. The intensity of CD29 and expression of ICAM-1 also increased
       on both CD4+CD45RO+ and CD8+CD45RO+ cells after culture with the CMV
       antigen. Up-regulation of adhesion molecules occurred on activated T
       cells by culture with the CMV antigen. This change was observed mainly
       on CD45RO+ T memory cells. The results suggested that these changes of
       adhesion molecules on activated T cells with CMV may contribute to some
       immune reaction or inflammatory change.
 DE    Adult  Antibodies, Monoclonal  Antigens, CD/BIOSYNTHESIS  Antigens,
       Viral/*IMMUNOLOGY  Cell Adhesion Molecules/*BIOSYNTHESIS  Cells,
       Cultured  Cytomegalovirus/*IMMUNOLOGY  CD4-Positive
       T-Lymphocytes/IMMUNOLOGY  CD8-Positive T-Lymphocytes/IMMUNOLOGY  Flow
       Cytometry  Human  Intercellular Adhesion Molecule-1/BIOSYNTHESIS
       Lymphocyte Function-Associated Antigen-1/BIOSYNTHESIS
       T-Lymphocytes/*IMMUNOLOGY  JOURNAL ARTICLE

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