Document 0445
 DOCN  M9550445
 TI    Quantitation of mycobacteria in blood specimens from patients with AIDS.
 DT    9505
 AU    Sanchez T; Vanderkolk J; Seay S; Heifets L; National Jewish Center for
       Immunology and Respiratory Medicine,; Denver, Colorado 80206.
 SO    Tuber Lung Dis. 1994 Oct;75(5):386-90. Unique Identifier : AIDSLINE
       MED/95143527
 AB    OBJECTIVE: To determine the effect of a delay in processing blood
       specimens, that may occur by mailing them to a specialized laboratory,
       on the quantitation of the number of mycobacteria. DESIGN: Simulated
       blood specimens in sodium polyanetholium sulfonate (SPS)-containing
       vacutainers were spiked with Mycobacterium avium, and stored for 4 days
       after addition of clarithromycin or rifabutin, or remained drug-free.
       Daily samples were lysed with sodium desoxycholate, and inoculated onto
       7H11 agar plates for the subsequent colony-forming units (CFU)/ml count.
       The isolation rates on two media, 7H11 agar and BACTEC 12B broth, were
       determined with 1212 specimens from AIDS patients. RESULTS: The number
       of bacteria in blood specimens remained without substantial change
       during the period of storage, even in the presence of clarithromycin or
       rifabutin, especially after refrigeration for 2 or 24 h before storage.
       7H11 agar and 12B broth were equally sensitive for isolation of M.
       avium. CONCLUSIONS: A 4-day delay in processing blood specimens in SPS
       vacutainers does not affect the results of quantitation of the number of
       mycobacteria. Combined use of 12B broth and 7H11 agar increased the
       overall recovery along with an opportunity to quantitate the number of
       CFU/ml.
 DE    AIDS-Related Opportunistic Infections/*MICROBIOLOGY  *Blood
       Preservation/METHODS  Clarithromycin/PHARMACOLOGY  Colony Count,
       Microbial  Culture Media  Dose-Response Relationship, Drug  Human
       Mycobacterium avium Complex/DRUG EFFECTS/GROWTH & DEVELOPMENT/
       *ISOLATION & PURIF  Mycobacterium avium-intracellulare
       Infection/*MICROBIOLOGY  Rifabutin/PHARMACOLOGY  Support, Non-U.S. Gov't
       Time Factors  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).