Document 0455
 DOCN  M9550455
 TI    Hybrid PCR sequencing: sequencing of PCR products using a universal
       primer.
 DT    9505
 AU    Berg ES; Olaisen B; Department of Virology, National Institute of Public
       Health,; Oslo, Norway.
 SO    Biotechniques. 1994 Nov;17(5):896-901. Unique Identifier : AIDSLINE
       MED/95143023
 AB    We describe a general method for making template DNA for sequencing of
       PCR products. The procedure may be particularly useful for PCR products
       where minimal sequence information is known or as an alternative to
       primer walking when sequencing long PCR products. A cassette containing
       the hybridization site for the M13 sequencing primer is ligated to a
       sample PCR product. Using one phosphorylated primer specific for the
       cassette together with one primer specific for the sample PCR product,
       subsequent PCR amplifies one hybrid construct directionally. This allows
       utilization of the universal M13 primer when sequencing of one strand
       after the removal of the complementary strand using lambda-exonuclease.
 DE    Astroviruses/GENETICS  Base Sequence  *DNA Primers  DNA Restriction
       Enzymes/METABOLISM  DNA, Viral/CHEMISTRY  HIV-1/GENETICS  Molecular
       Sequence Data  *Nucleic Acid Hybridization  Phosphorylation  *Polymerase
       Chain Reaction  Sequence Analysis, DNA/*METHODS  Templates  TECHNICAL
       REPORT  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).