Document 0490
 DOCN  M9550490
 TI    Small amino acid sequence changes within the V2 domain can affect the
       function of a T-cell line-tropic human immunodeficiency virus type 1
       envelope gp120.
 DT    9505
 AU    Koito A; Stamatatos L; Cheng-Mayer C; Department of Medicine, University
       of California, San Francisco; 94143-0128.
 SO    Virology. 1995 Feb 1;206(2):878-84. Unique Identifier : AIDSLINE
       MED/95159441
 AB    Prior studies with recombinant viruses constructed in vitro showed that
       the V2 domain of envelope gp120, in addition to the required V3 domain,
       enhances the efficiency of infection of primary macrophages by HIV-1.
       Present structural studies on the gp120s of these recombinant viruses
       using three human monoclonal antibodies directed to the V3 loop indicate
       that the V2 domain affects cell tropism by modulating the conformation
       of the V3 loop. Additional mutational analyses of the V2 domain of the
       T-cell line-tropic virus HIV-1SF2 reveal that single amino acid sequence
       changes, mainly those affecting the location of potential N-linked
       glycosylation sites and the positive charge of this region, can also
       alter tropism. These amino acid substitutions in the V2 domain, however,
       do not appear to alter the conformation of the V3 loop. Thus, the V2
       domain of gp120 can influence cell tropism through both an effect on V3
       as well as via a V3-independent mechanism.
 DE    Amino Acid Sequence  Animal  Antibodies, Monoclonal  Cells, Cultured
       Cercopithecus aethiops  Comparative Study  Conserved Sequence
       Glycosylation  Human  HIV Envelope Protein
       gp120/BIOSYNTHESIS/CHEMISTRY/*PHYSIOLOGY
       HIV-1/*PHYSIOLOGY/PATHOGENICITY  Kidney  Kinetics  Molecular Sequence
       Data  Mutagenesis, Site-Directed  Recombinant
       Proteins/BIOSYNTHESIS/CHEMISTRY/METABOLISM  Sequence Homology, Amino
       Acid  Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.
       T-Lymphocytes/*VIROLOGY  Transfection  *Virus Replication  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).