Document 0491
 DOCN  M9550491
 TI    Overexpression of human immunodeficiency virus type 1 protease increases
       intracellular cleavage of Gag and reduces virus infectivity.
 DT    9505
 AU    Luukkonen BG; Fenyo EM; Schwartz S; Microbiology and Tumorbiology
       Center, Karolinska Institute,; Stockholm, Sweden.
 SO    Virology. 1995 Feb 1;206(2):854-65. Unique Identifier : AIDSLINE
       MED/95159439
 AB    Here we have investigated if human immunodeficiency virus type 1 (HIV-1)
       protease expressed in trans can interfere with production of infectious
       HIV-1 particles. Protease produced from a Tat and Rev inducible
       expression plasmid specifically cleaved HIV-1 p55Gag in a dose-dependent
       manner. Coexpression of protease and an infectious HIV-1 proviral clone
       resulted in increased intracellular cleavage of p55Gag. As a
       consequence, virus production and virus infectivity was significantly
       reduced. These results suggest that overexpression of HIV-1 protease in
       HIV-1-infected cells is a powerful way to inhibit production of
       infectious virions.
 DE    Base Sequence  Cell Line  Cells, Cultured  DNA Primers  Gene Expression
       Gene Products, gag/*METABOLISM  Human  HIV Core Protein p24/BIOSYNTHESIS
       HIV Protease/*BIOSYNTHESIS/METABOLISM  HIV-1/*PHYSIOLOGY/PATHOGENICITY
       Kinetics  Leukocytes, Mononuclear/VIROLOGY  Molecular Sequence Data
       Mutagenesis, Site-Directed  Plasmids  Polymerase Chain Reaction
       Recombinant Proteins/BIOSYNTHESIS/METABOLISM  Support, Non-U.S. Gov't
       Transfection  *Virus Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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