Document 0740
 DOCN  M9550740
 TI    Viral DNA burden and decline in percentage of CD4-positive cells in the
       lymphoid compartment of SIV-infected macaques.
 DT    9505
 AU    Rosenberg YJ; Lewis MG; Leon EC; Cafaro A; Eddy GA; Greenhouse JJ; Henry
       M. Jackson Foundation Laboratories, Rockville, Maryland; 20850.
 SO    AIDS Res Hum Retroviruses. 1994 Oct;10(10):1269-77. Unique Identifier :
       AIDSLINE MED/95151364
 AB    The decline in CD4+ cells and increased viral DNA and RNA burden in the
       blood of human immunodeficiency virus (HIV)-infected individuals have
       been used as closely related correlates of disease progression. However,
       little is known about levels of total or unintegrated viral DNA in
       lymphoid tissue of HIV-infected patients and how they relate to CD4+
       cell decline or disease progression. Exploiting the similarities between
       HIV- and simian immunodeficiency virus (SIV)-induced disease, we
       examined lymphoid organs and peripheral blood from SIV-infected macaques
       for total (pol) and unintegrated 2-LTR circular viral DNA by polymerase
       chain reaction (PCR). Two SIV isolates (SIVmac/251 and SIVmne/E11S) that
       differ markedly in their biological and clinical properties were
       studied. The results indicate that total viral DNA burdens vary
       considerably between isolates. There was no strong association between
       total viral DNA levels and CD4% in lymphoid tissues when isolates were
       compared and death was not associated with any particular level of viral
       pol DNA. In contrast, accumulation of unintegrated viral DNA was closely
       associated with decline in CD4/CD8 ratios in lymphoid organs and AIDS.
       The appearance of both pol and unintegrated viral DNA in thymus of
       infected macaques also emerged as one of the single best correlates or
       possible predictors of advanced disease yet studied. Their roles in
       pathogenesis are discussed.
 DE    Animal  Antibodies, Viral/BLOOD  Base Sequence  Comparative Study  CD4
       Lymphocyte Count  CD4-CD8 Ratio  CD4-Positive T-Lymphocytes/*VIROLOGY
       DNA Primers  DNA, Viral/*ANALYSIS/GENETICS  Genes, pol  Human
       HIV/GENETICS  HIV Infections/IMMUNOLOGY/VIROLOGY  Macaca  Molecular
       Sequence Data  Polymerase Chain Reaction  Repetitive Sequences, Nucleic
       Acid  Simian Acquired Immunodeficiency Syndrome/*IMMUNOLOGY/*VIROLOGY
       SIV/GENETICS/ISOLATION & PURIF/*PHYSIOLOGY  Thymus Gland/VIROLOGY  Virus
       Integration  JOURNAL ARTICLE

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