Document 0804
 DOCN  M9550804
 TI    Loss of red blood cell viability associated with limited thermal
       inactivation of extracellular HIV-1.
 DT    9505
 AU    Stromberg RR; Kuypers FA; Sawyer L; Friedman LI; Cole M; Tran K; Hanson
       CV; Product Development Department, Holland Laboratory, American Red;
       Cross, Rockville, MD 20855.
 SO    Vox Sang. 1994;67(3):260-6. Unique Identifier : AIDSLINE MED/95167804
 AB    The effects of incubation at mildly elevated temperatures on HIV-1
       inactivation and in vitro red blood cell properties were investigated.
       Red cells (55% Hct) were leukodepleted (3 log10) by filtration,
       maintained at 45 or 47 degrees C for 4 or 8 h, and then stored at 4
       degrees C. Hemolysis was twice that of controls after 42-day storage for
       samples treated for 4 h at 45 degrees C, and five times larger for
       samples heated at 47 degrees C. There was also a significant increase in
       the rate of potassium loss, an early decrease in ATP levels, and an
       initial drop in pH for samples treated at either temperature. Larger
       differences were observed for samples exposed to these elevated
       temperatures for 8 h. Osmotic deformability curves obtained by
       ektacytometry showed dramatic decreases in red cell deformability at
       both temperatures and for both time periods. HIV-1 inactivation in red
       cells treated at 45 degrees C (approximately 0.25 log10/h) was
       considerably less than that obtained in tissue culture medium (1-2
       log10/h). Since the decrease in red cell deformability is likely to
       indicate reduced red cell function and survival, and the rate of HIV-1
       inactivation is low, mild heat treatment is not an adequate process for
       viral inactivation of red cell products.
 DE    Blood/VIROLOGY  Blood Preservation/*METHODS  Erythrocyte Deformability
       *Erythrocytes  *Heat  Hemolysis  Human  Hydrogen-Ion Concentration
       *HIV-1  Osmotic Fragility  JOURNAL ARTICLE

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