Document 0831
 DOCN  M9550831
 TI    Heterogeneity of antigen-specific CD4+ T cell clones from a patient with
       Schistosomiasis mansoni.
 DT    9505
 AU    Hirayama K; Abrams JS; Quinn JJ; Harn DA; Department of Tropical Public
       Health, Harvard School of Public; Health, Boston, MA 02115.
 SO    Parasite Immunol. 1994 Nov;16(11):561-9. Unique Identifier : AIDSLINE
       MED/95166573
 AB    Two subsets of differentiated murine helper T cells, Th1 and Th2, based
       on secretion products in response to antigen have been described (Cher &
       Mosmann 1987, Coffman et al. 1988, Lopez et al. 1988, Paliard et al.
       1988, Patel et al. 1988, Mosmann & Coffman 1989). To analyse
       immunological function of antigen-specific CD4+T cells in human
       schistosomiasis, we produced schistosomal egg antigen-specific T cell
       clones from a former patient. We identified four different types of CD4+
       T cell clones by analysis of cytokine production. Two of the four types
       of the clones corresponded to murine Th1 or Th2 subsets; a third type
       was of the Th0 subset (Th1 + 2) and a fourth type produced IL-5
       dissociated from IL-4. Analysis of the antigen(s) recognized by these T
       cell clones showed that all of the clones proliferated in response to
       soluble egg antigen(s) (SEA) found within a pl fraction whose pH was
       5.2. T cell Western blot analysis of the stimulatory pl fraction
       demonstrated that the apparent Mr of the relevant antigens recognized by
       the clones were 38 kDa for the Th2 homologue, and 45-55 kDa for the Th1
       homologue.
 DE    Animal  Antigens, Protozoan/*IMMUNOLOGY  Antigens, Surface/ANALYSIS
       Base Sequence  Clone Cells  Cytokines/BIOSYNTHESIS  CD4-Positive
       T-Lymphocytes/*IMMUNOLOGY  DNA Primers  Human  In Vitro  Mice  Molecular
       Sequence Data  Ovum  RNA, Messenger  Schistosomiasis/*IMMUNOLOGY
       Schistosomiasis mansoni/IMMUNOLOGY  Support, Non-U.S. Gov't  Th1
       Cells/IMMUNOLOGY  Th2 Cells/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).