Document 0882
 DOCN  M9550882
 TI    Immunopathological mechanisms of human T cell lymphotropic virus type 1
       (HTLV-I) uveitis. Detection of HTLV-I-infected T cells in the eye and
       their constitutive cytokine production.
 DT    9505
 AU    Sagawa K; Mochizuki M; Masuoka K; Katagiri K; Katayama T; Maeda T;
       Tanimoto A; Sugita S; Watanabe T; Itoh K; Department of Immunology,
       Kurume University School of Medicine,; Japan.
 SO    J Clin Invest. 1995 Feb;95(2):852-8. Unique Identifier : AIDSLINE
       MED/95164721
 AB    The immunopathology of human T cell lymphotropic virus type 1 (HTLV-I)
       uveitis was addressed by using T cell clones (TCC) established from the
       intraocular fluid of patients with HTLV-I uveitis. Proviral DNA of
       HTLV-I was identified in 55 out of 94 (59%) or 13 out of 36 (36%) TCC
       from the ocular fluid or the peripheral blood of these patients,
       respectively. Most of HTLV-I-infected TCC had a CD3+ CD4+ CD8-
       phenotype. HTLV-I infection on TCC was confirmed by analysis of the
       viral mRNA, nucleotide sequence, virus-associated proteins, and virus
       particles. HTLV-I-infected TCC, but not HTLV-I negative TCC,
       constitutively produced high amounts of IL-6 (1,336 +/- 1,050 pg/ml) and
       TNF-alpha (289 +/- 237 pg/ml) in the absence of any stimuli.
       HTLV-I-infected TCC from the ocular lesion also constitutively produced
       high amounts of IL-1 alpha (12,699 pg/ml), IL-2 (61 pg/ml), IL-3 (428
       pg/ml), IL-8 (1,268 pg/ml), IL-10 (28 pg/ml), IFN-gamma (5,095 pg/ml),
       and GM-CSF (2,886 pg/ml). Hydrocortisone, a drug effective in vivo for
       the treatment of HTLV-I uveitis, severely depressed cytokine production
       in vitro in most cases. In summary, the results demonstrated direct
       evidence of HTLV-I infection of the eye and suggest that cytokines
       produced by HTLV-I-infected T cells are responsible for the intraocular
       inflammation in patients with HTLV-I uveitis.
 DE    Adult  Aqueous Humor/IMMUNOLOGY  Base Sequence  Behcet's
       Syndrome/IMMUNOLOGY/PATHOLOGY/VIROLOGY  Case Report
       Cytokines/*BIOSYNTHESIS  DNA Primers  Female  Gene Expression/DRUG
       EFFECTS  Genes, env  Genes, pX  Granulocyte-Macrophage
       Colony-Stimulating Factor/BIOSYNTHESIS  Human
       Hydrocortisone/PHARMACOLOGY  HTLV-I/GENETICS/*ISOLATION &
       PURIF/ULTRASTRUCTURE  HTLV-I Infections/*IMMUNOLOGY/*PATHOLOGY/VIROLOGY
       Interferon Type II/BIOSYNTHESIS  Interleukins/BIOSYNTHESIS  Male
       Microscopy, Electron  Middle Age  Molecular Sequence Data  Polymerase
       Chain Reaction  RNA, Messenger/ANALYSIS  RNA, Viral/ANALYSIS  Support,
       Non-U.S. Gov't  T-Lymphocytes/*IMMUNOLOGY/PATHOLOGY
       Uveitis/*IMMUNOLOGY/*PATHOLOGY/VIROLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).