Document 0885
 DOCN  M9550885
 TI    The cutaneous T cell lymphoma, mycosis fungoides, is a human T cell
       lymphotropic virus-associated disease. A study of 50 patients.
 DT    9505
 AU    Pancake BA; Zucker-Franklin D; Coutavas EE; Department of Medicine, New
       York University Medical Center, New; York 10016.
 SO    J Clin Invest. 1995 Feb;95(2):547-54. Unique Identifier : AIDSLINE
       MED/95164683
 AB    For nearly two decades it has been suspected that the cutaneous T cell
       lymphoma, mycosis fungoides (MF), and its leukemic variant, the Sezary
       syndrome, are caused by the human T lymphotropic virus (HTLV-I/II).
       Arguments against this concept included the finding that only a small
       number of MF patients have antibodies to HTLV-I/II and that attempts to
       detect proviral sequences by mere Southern hybridization of extracted
       DNA usually met with failure. However, we have reported repeatedly that
       HTLV-like particles emerge in blood mononuclear cell (PBMC) cultures of
       practically all patients with this disease. In several instances, the
       particles were identified as HTLV by immunoelectron microscopy as well
       as biomolecular analysis. With the assumptions that the virus in MF
       patients may have become detection by Southern hybridization alone, the
       extracts of freshly isolated PBMC of 50 consecutive patients were
       subjected to combined PCR/Southern analysis. Here we report the presence
       of HTLV pol and/or tax proviral sequences in 46 out of 50 (92%) of the
       patients tested. In addition, five of the patients, who lacked
       antibodies to HTLV-I/II structural proteins, were found to be
       seropositive for tax. It thus seems reasonable to conclude that
       MF/Sezary syndrome is an HTLV-associated disease and that lack of an
       immune response does not preclude infection with this type of virus.
 DE    Adult  Aged  Aged, 80 and over  Amino Acid Sequence  Base Sequence
       Cells, Cultured  Child  Cloning, Molecular  Comparative Study  DNA
       Primers  DNA, Viral/ANALYSIS/*BLOOD  Female  Gene Products,
       tax/*BIOSYNTHESIS  Genes, pol  Genes, pX  Genes, Viral  Human
       HTLV-I/*ISOLATION & PURIF/PATHOGENICITY/ULTRASTRUCTURE
       Lymphocytes/*VIROLOGY  Male  Microscopy, Electron  Middle Age  Molecular
       Sequence Data  Mycosis Fungoides/*VIROLOGY  Polymerase Chain Reaction
       Reference Values  Sequence Homology, Nucleic Acid  Sezary
       Syndrome/*VIROLOGY  Skin Neoplasms/*VIROLOGY  Support, Non-U.S. Gov't
       Support, U.S. Gov't, P.H.S.  CLINICAL TRIAL  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).