Document 0972
 DOCN  M9570972
 TI    AIDS-related Kaposi's sarcoma is a clonal neoplasm.
 DT    9506
 AU    Rabkin CS; Bedi G; Musaba E; Sunkutu R; Mwansa N; Sidransky D; Biggar
       RJ; Viral Epidemiology Branch, NCI, EPN/434, Bethesda, MD 20892
 SO    Clin Cancer Res; 1(3):257-60 1995. Unique Identifier : AIDSLINE
       ICDB/95615095
 AB    Kaposi's sarcoma is generally believed to be a non-neoplastic
       hyperproliferation because it may regress spontaneously and its spindle
       cells lack features of typical tumor cells, such as aneuploidy, nuclear
       atypia, and permissive growth in cell culture. A fundamental
       characteristic of neoplasms is clonality, in that they arise from clonal
       replication of a single cell whereas reactive processes are derived from
       polyclonal proliferation. We used an X chromosome inactivation assay to
       determine the clonality of Kaposi's sarcoma nodules from patients with
       AIDS-related disease. The assay is based on a methyl-sensitive
       restriction digest followed by PCR amplification of the highly
       polymorphic androgen receptor gene. Two of three evaluable cases had a
       monoclonal pattern of inactivation, and the third case had a clonal
       expansion of cells with an altered microsatellite repeat sequence. These
       data suggest that Kaposi's sarcoma (at least in the AIDS setting) is a
       clonal neoplasm. (19 Refs)
 DE    Acquired Immunodeficiency Syndrome/*COMPLICATIONS  Aneuploidy  Cell
       Division  DNA, Satellite  Dosage Compensation (Genetics)  Human
       Polymerase Chain Reaction  Polymorphism (Genetics)  Receptors,
       Androgen/GENETICS  Repetitive Sequences, Nucleic Acid  Sarcoma,
       Kaposi's/GENETICS/*PATHOLOGY  Tumor Cells, Cultured  JOURNAL ARTICLE

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