Document 0058
 DOCN  M9580058
 TI    Cellular responses during liver fluke infection in sheep and its evasion
       by the parasite.
 DT    9506
 AU    Meeusen E; Lee CS; Rickard MD; Brandon MR; Centre for Animal
       Biotechnology, University of Melbourne,; Parkville, Victoria, Australia.
 SO    Parasite Immunol. 1995 Jan;17(1):37-45. Unique Identifier : AIDSLINE
       MED/95249295
 AB    The cellular immune response in sheep to an acute and chronic primary
       and an acute secondary liver fluke infection were examined by
       immunohistology of liver tissue and flowcytometry of lymphocytes from
       the draining hepatic lymph nodes. Ten days after primary infection,
       portal tract areas surrounding migratory tunnels were infiltrated with
       CD4+ and CD8+ lymphocytes with fewer B cells and T19+ T cells. Micro
       abscesses were distributed sporadically in the liver parenchyma and
       young flukes could be easily observed in the liver tissue free from
       inflammatory cells. More intensive infiltration of the portal tract
       areas was observed during a secondary liver fluke infection
       characterized by a pronounced increase in eosinophils, B cells and CD4+
       T cells. In addition, there was an increase in MHC class II+
       fibroblastic-like cells surrounding the migratory tracts. In contrast to
       the primary infection, no young flukes were observed in the same tissue
       areas during the secondary infection. Chronic primary infections were
       characterized by perilobular fibrosis and a predominance of CD8+ and
       gamma delta-TCR+T19- T cells distributed within fibrotic strands.
       Distinct B cell follicles were observed in the fibrotic strands and near
       major bile ducts and necrotic patches. Pronounced lymphocyte
       infiltration could occasionally be observed surrounding liver fluke eggs
       lodged in liver tissue. A progressive increase in lymph node weight,
       cell number and CD4/CD8 ratio was observed in the acute and chronic
       primary infections. The role of the infiltrating cell populations and
       possible mechanism of immune evasion by the parasite are discussed.
 DE    Animal  Antigens, CD4/ANALYSIS  Antigens, CD45/ANALYSIS  Antigens,
       CD8/ANALYSIS  CD4-CD8 Ratio  Fasciola hepatica/*IMMUNOLOGY
       Fascioliasis/*IMMUNOLOGY/PATHOLOGY  Histocompatibility Antigens Class
       II/ANALYSIS  Immunity, Cellular  Sheep  JOURNAL ARTICLE

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