Document 0059
 DOCN  M9580059
 TI    Cytotoxicity in human mucosal and cutaneous leishmaniasis.
 DT    9506
 AU    Barral-Netto M; Barral A; Brodskyn C; Carvalho EM; Reed SG; Universidade
       Federal da Bahia, Salvador-Bahia, Brazil.
 SO    Parasite Immunol. 1995 Jan;17(1):21-8. Unique Identifier : AIDSLINE
       MED/95249293
 AB    CD8+ T cells and lysis of parasitized macrophages seem to be important
       in the resistance to murine leishmaniasis. In the present study, we
       evaluated peripheral blood mononuclear cell (PBMC) from patients with
       either cutaneous (CL) or mucosal (ML) leishmaniasis in cell lysis assays
       using 51-Cr-labeled Daudi or K562 cells, or autologous antigen-pulsed
       macrophages as targets. Results are reported as lytic units (number of
       cells required for 30% lysis) per million PBMC. Exposure of patient PBMC
       (n = 12) to lysate from Leishmania amazonensis promastigotes led to an
       increase in cytotoxic activity compared to unstimulated patient cells
       against Daudi (81.8 +/- 14.9 vs 13.6 +/- 5 lytic units (LU) per million
       PBMC; mean +/- SEM) and K562 (65.7 +/- 8.4 vs 13.1 +/- 5 LU/10(6) PBMC).
       ML had higher responses than CL in both targets (80.4 +/- 11.0 vs 46.4
       +/- 11.6 LU/10(6) PBMC for K562, and 104.3 +/- 23.8 vs 59.3 +/- 14.3
       LU/10(6) PBMC for Daudi). Normal control PBMC, stimulated with L.
       amazonensis antigen had 6.32 +/- 3.72 LU/10(6) PBMC against Daudi cells
       and 9.06 +/- 2.78 LU/10(6) PBMC against K562. The cell responsible for
       lysis of the K562 cells was characterized as NK, by means of cell
       separation employing magnetic beads coupled to antibodies. Addition of
       recombinant TGF-beta or recombinant human IL-10 reduced L.
       amazonensis-induced cytotoxicity by 90% and 70%, respectively.
       Cytotoxicity of antigen-stimulated PBMC was also demonstrated against
       autologous L. amazonensis antigen-pulsed macrophages in the range of 6.7
       to 41.7 LU/10(6) PBMC.(ABSTRACT TRUNCATED AT 250 WORDS)
 DE    Animal  Antibodies, Protozoan/IMMUNOLOGY  Antigens, Protozoan/IMMUNOLOGY
       *Cytotoxicity, Immunologic  CD4-Positive T-Lymphocytes/IMMUNOLOGY
       CD8-Positive T-Lymphocytes/*IMMUNOLOGY  Dose-Response Relationship,
       Immunologic  Human  Interferon Type II/IMMUNOLOGY
       Interleukin-10/IMMUNOLOGY  Killer Cells/IMMUNOLOGY  Leishmania
       mexicana/*IMMUNOLOGY  Leishmaniasis, Cutaneous/*IMMUNOLOGY/PARASITOLOGY
       Leukocytes, Mononuclear/IMMUNOLOGY  Macrophages/IMMUNOLOGY  Mice
       Support, U.S. Gov't, P.H.S.  T-Lymphocytes, Cytotoxic  Transforming
       Growth Factor beta/IMMUNOLOGY  Tumor Cells, Cultured/IMMUNOLOGY  JOURNAL
       ARTICLE

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