Document 0083
 DOCN  M9580083
 TI    The humanized severe combined immunodeficient mouse as a model for
       primary human humoral response against HIV1 peptides.
 DT    9506
 AU    Chargui J; Dye D; Blomberg J; Desgranges C; Touraine JL; INSERM U80,
       Hopital Edouard Herriot, Lyon, France.
 SO    J Immunol Methods. 1995 Apr 12;181(1):91-100. Unique Identifier :
       AIDSLINE MED/95248141
 AB    Adequate animal models for the study of human immunodeficiency virus
       (HIV) infection are important for the analysis of specific cellular and
       humoral immune responses. Humanized severe combined immunodeficiency
       (SCID) mice can be constructed either by injecting human peripheral
       blood lymphocytes (hu-PBL-SCID) or by transplanting human fetal
       tissues--liver, thymus and bone fragments--(SCID-hu) into these mice.
       Such animals can produce human immunoglobulins and SCID-hu mice exhibit
       circulating T and B lymphocytes of human origin. These humanized mice
       were injected with immunogenic HIV peptides and the specific humoral
       response was studied. A human antibody response was obtained after de
       novo contact with HIV1 peptides p583 and p642, from gp41. In SCID-hu
       mice, a primary, then a secondary response were demonstrated to occur
       with 225 mg/l of human immunoglobulin (Ig)M and 300-1860 mg/l human IgG.
       When tested in ELISA, these human antibodies recognized specifically
       both the immunization peptides and the HIV1 antigens. The antibody
       response was obviously of a primary nature since the human cells derived
       from naive fetal cells. When SCID mice received intraperitoneal
       injections of human peripheral blood lymphocytes pre-incubated in vitro
       with peptide p583 for 1 week, and when the resulting hu-PBL-SCID mice
       were injected with the same peptide, only IgM anti-HIV antibodies were
       produced (372-424 mg/l) and the switch to IgG antibodies did not occur.
       This model may provide a means to produce human monoclonal antibodies to
       HIV and to check candidate HIV vaccines.
 DE    Amino Acid Sequence  Animal  B-Lymphocytes/IMMUNOLOGY  Disease Models,
       Animal  Fetal Tissue Transplantation/IMMUNOLOGY  Human  HIV
       Antibodies/*BIOSYNTHESIS  HIV Envelope Protein gp41/*IMMUNOLOGY
       HIV-1/*IMMUNOLOGY  Immunization  Mice  Mice, SCID  Molecular Sequence
       Data  Peptide Fragments/CHEMISTRY/*IMMUNOLOGY  Severe Combined
       Immunodeficiency/*IMMUNOLOGY  T-Lymphocytes/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).