Document 0085
 DOCN  M9580085
 TI    Early detection of apoptosis in defined lymphocyte populations in vivo.
 DT    9506
 AU    Zhang L; Wang C; Radvanyi LG; Miller RG; Ontario Cancer Institute,
       Toronto, Canada.
 SO    J Immunol Methods. 1995 Apr 12;181(1):17-27. Unique Identifier :
       AIDSLINE MED/95248133
 AB    Recently many methods have been developed for the detection of
       apoptosis. However, all of them have some limitations in determining
       whether specific subsets of cells are undergoing apoptosis. In this
       paper we describe a technique in which one simultaneously stains for
       cell surface markers with fluorescent monoclonal antibodies and for
       nuclear DNA breaks using in situ DNA nick translation detectable by
       fluorescence. The method has been evaluated using radiation-induced
       programmed cell death of lymphocytes and compared with some other
       techniques. It was found that the method is very specific and sensitive.
       It enabled us to enumerate apoptotic cells at the single cell level and
       simultaneously determine their subset-specific surface antigen profile
       both in vivo and in vitro. It is also insensitive to nicks present in
       replicating cells. Our data suggest that this method may be useful for
       the study of programmed cell death of antigen specific T cells in vivo.
 DE    Animal  Antibodies, Monoclonal  Apoptosis/*PHYSIOLOGY/RADIATION EFFECTS
       Cell Death/PHYSIOLOGY  CD4-Positive T-Lymphocytes/*PHYSIOLOGY
       CD8-Positive T-Lymphocytes/*PHYSIOLOGY  DNA/ANALYSIS  DNA
       Damage/PHYSIOLOGY  DNA Replication  Electrophoresis, Agar Gel  Flow
       Cytometry  Lymphocyte Subsets  Mice  Mice, Inbred C57BL  T-Lymphocytes,
       Cytotoxic/PHYSIOLOGY  JOURNAL ARTICLE

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