Document 0087
 DOCN  M9580087
 TI    Analysis of apoptosis in lymph nodes of HIV-infected persons. Intensity
       of apoptosis correlates with the general state of activation of the
       lymphoid tissue and not with stage of disease or viral burden.
 DT    9506
 AU    Muro-Cacho CA; Pantaleo G; Fauci AS; Laboratory of Immunoregulation,
       National Institute of Allergy and; Infectious Diseases, National
       Institutes of Health, Bethesda, MD; 20892, USA.
 SO    J Immunol. 1995 May 15;154(10):5555-66. Unique Identifier : AIDSLINE
       MED/95248123
 AB    The occurrence of in vivo apoptosis was investigated in lymph node
       sections obtained from HIV-infected persons at different stages of
       disease. The degree of apoptosis in lymph nodes from HIV-infected
       individuals was compared with that observed in lymph nodes obtained from
       HIV-negative individuals. Apoptosis was readily detected in lymph nodes
       obtained from both HIV-negative and HIV-positive persons; however, the
       degree of apoptosis in lymph nodes obtained from HIV-positive persons
       was three to four times higher than that observed in the lymph nodes
       obtained from HIV-negative persons. In contrast to HIV-negative lymph
       nodes in which apoptosis was confined largely to germinal centers, in
       HIV-positive lymph nodes all functional compartments of the lymph node
       (i.e., cortex, paracortex, and sinuses) were extensively involved by
       this phenomenon. Furthermore, a significant correlation was observed
       between intensity of apoptosis and degree of activation of the lymphoid
       tissue associated with HIV infection. In contrast, intensity of
       apoptosis correlated neither with the clinical stage of HIV disease nor
       with the viral burden in the lymph node. Finally, apoptosis was not
       restricted only to CD4+ T cells; both B cells and CD8+ T cells were
       found to undergo apoptosis. Taken together, these results indicate that
       the increased intensity of the apoptotic phenomenon in HIV infection is
       caused by the general state of immune activation, and is independent of
       the progression of HIV disease and of the levels of viral load.
 DE    Acridine Orange  Apoptosis/*IMMUNOLOGY  DNA Damage  DNA, Viral/ANALYSIS
       Electrophoresis, Agar Gel  Human  HIV Infections/*IMMUNOLOGY/VIROLOGY
       HIV-1/IMMUNOLOGY/ISOLATION & PURIF  Leukocytes, Mononuclear/IMMUNOLOGY
       Lymph Nodes/CYTOLOGY/*IMMUNOLOGY/VIROLOGY  Polymerase Chain Reaction
       JOURNAL ARTICLE

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