Document 0092
 DOCN  M9580092
 TI    Growth inhibition of Candida albicans hyphae by CD8+ lymphocytes.
 DT    9506
 AU    Beno DW; Stover AG; Mathews HL; Department of Microbiology and
       Immunology, Stritch School of; Medicine, Loyola University of Chicago,
       Maywood, IL 60153, USA.
 SO    J Immunol. 1995 May 15;154(10):5273-81. Unique Identifier : AIDSLINE
       MED/95248093
 AB    We have shown previously that IL-2-activated splenocytes can inhibit the
       growth of Candida albicans hyphae in vitro. Herein we demonstrate that
       plastic nonadherent lymphocytes that are CD8+ mediate the antifungal
       activity. Enrichment for CD8+ cells markedly enhanced the antifungal
       activity of the IL-2-activated lymphocyte population for C. albicans and
       the cytotoxic activity of the lymphocytes for an NK-resistant cell line.
       Depletion of CD8+ cells reduced the lymphocyte population's antifungal
       activity and cytotoxic activity for the NK-resistant cell line.
       Enrichment for NK1.1+ cells markedly reduced the antifungal activity of
       the lymphocyte population for C. albicans and increased the cytotoxic
       activity of the lymphocytes for an NK-sensitive cell line. Depletion of
       NK1.1+ cells increased the lymphocyte population's antifungal activity
       and cytotoxic activity for the NK-resistant cell line. Generation of the
       antifungal lymphocytes in culture required IL-2 and was not replaced
       with IFN-gamma. These data show that IL-2-activated CD8+ T lymphocytes
       exert the greatest amount of antifungal effect against the hyphal form
       of C. albicans, whereas IL-2- or IFN-gamma-activated NK cells have
       little or no effect against the hyphae.
 DE    Animal  Candida albicans/CYTOLOGY/*GROWTH & DEVELOPMENT/*IMMUNOLOGY
       Complement/PHYSIOLOGY  Cytotoxicity Tests, Immunologic  CD8-Positive
       T-Lymphocytes/*IMMUNOLOGY  Female  Flow Cytometry
       Interleukin-2/PHARMACOLOGY  Killer Cells, Natural/IMMUNOLOGY  Mice
       Mice, Inbred C57BL  Recombinant Proteins/PHARMACOLOGY
       Spleen/CYTOLOGY/DRUG EFFECTS  Support, Non-U.S. Gov't  Support, U.S.
       Gov't, P.H.S.  Tumor Cells, Cultured  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).