Document 0094
 DOCN  M9580094
 TI    Dendritic cells produce IL-12 and direct the development of Th1 cells
       from naive CD4+ T cells.
 DT    9506
 AU    Macatonia SE; Hosken NA; Litton M; Vieira P; Hsieh CS; Culpepper JA;
       Wysocka M; Trinchieri G; Murphy KM; O'Garra A; Department of Immunology,
       DNAX Research Institute of Molecular; and Cellular Biology Inc., Palo
       Alto, CA 94303, USA.
 SO    J Immunol. 1995 May 15;154(10):5071-9. Unique Identifier : AIDSLINE
       MED/95248071
 AB    Dendritic cells are APCs that are unique in their potency to stimulate
       proliferation of primary Ag-specific responses in vitro and in vivo. In
       this study, we demonstrate that dendritic cells can produce IL-12, a
       dominant cytokine involved in the development of IFN-gamma-producing T
       cells. This finding resulted from our observations that dendritic
       cell-induced Th1 development from total CD4+ T cells upon neutralization
       of endogenous levels of IL-4 was IL-12-dependent. Furthermore, we
       demonstrate that dendritic cells can induce the development of Th1 cells
       from Ag-specific naive LECAM-1bright CD4+ T cells obtained from alpha
       beta-TCR transgenic mice, provided that CD4+ LECAM-1dull T cells, which
       produce significant levels of IL-4, are not present in the primary
       cultures. Production of IL-12 by dendritic cells was confirmed by
       positive immunofluoresence staining with Abs specific for the inducible
       IL-12 p40 subunit. This suggests that in addition to inducing
       proliferation and clonal expansion of naive T cells, dendritic cells, by
       their production of IL-12, play a direct role in the development of
       IFN-gamma-producing cells that are important for cell-mediated immune
       responses.
 DE    Animal  Cell Differentiation/*IMMUNOLOGY  Cells, Cultured  CD4-Positive
       T-Lymphocytes/*IMMUNOLOGY  Dendritic Cells/*IMMUNOLOGY  Female
       Fluorescent Antibody Technique  Interleukin-12/*BIOSYNTHESIS/IMMUNOLOGY
       Interleukin-4/IMMUNOLOGY  Mice  Mice, Inbred BALB C  Mice, Transgenic
       Receptors, Antigen, T-Cell, alpha-beta/GENETICS  Support, Non-U.S. Gov't
       Th1 Cells/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).