Document 0097
 DOCN  M9580097
 TI    Identification of HTLV-I- or HTLV-II-producing cells by cocultivation
       with BHK-21 cells stably transfected with a LTR-lacZ gene construct.
 DT    9506
 AU    Astier-Gin T; Portail JP; Lafond F; Guillemain B; INSERM Unite 328,
       Bordeaux, France.
 SO    J Virol Methods. 1995 Jan;51(1):19-29. Unique Identifier : AIDSLINE
       MED/95247866
 AB    The Syrian Hamster kidney cell line (BHK-21) was stably transfected with
       a plasmid vector containing the lacZ bacterial gene under the control of
       a HTLV-I-LTR promoter. In these cells termed pA18G-BHK-21, this lacZ
       construct is inducible by the tax protein produced by a tax expression
       vector. It was also shown that beta-galactosidase synthesis was detected
       within 48 h after cocultivation of pA18G-BHK-21 cells with HTLV-I
       (HUT-102, MT2, C91/PL, 2060) or HTLV-II (MoT strain) -producing cells.
       The number of positive cells was directly related to the number of
       HTLV-I or -II-infected cells seeded. In addition, the LTR
       transactivation observed in coculture with HTLV-I-infected cells was
       specifically inhibited by sera containing antibodies directed against
       HTLV-I proteins, but not, or only weakly, by sera containing HTLV-II
       antibodies. Conversely, beta-galactosidase expression induced by
       HTLV-II-infected cells was inhibited by sera of HTLV-II-infected
       individuals, but not, or only weakly, by HTLV-I-positive sera.
       Irrespective of the inducer cell, sera from uninfected people did not
       inhibit LTR-driven expression of the lacZ gene in pA18G-BHK-21 cells
       cocultivated with HTLV-producing cells. This assay may thus be employed
       profitably for the detection and quantification of both HTLV-producing
       cells and HTLV-specific antibodies.
 DE    beta-Galactosidase/BIOSYNTHESIS/GENETICS  Animal  Cell Line  Genetic
       Vectors  Hamsters  Human  HTLV-I/*GENETICS/*PHYSIOLOGY  HTLV-I
       Antibodies/BLOOD  HTLV-II/*PHYSIOLOGY  HTLV-II Antibodies/BLOOD  Lac
       Operon  Neutralization Tests  Plasmids/GENETICS  Promoter Regions
       (Genetics)  Repetitive Sequences, Nucleic Acid  Support, Non-U.S. Gov't
       Trans-Activation (Genetics)  Transfection  Virology/METHODS  Virus
       Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).