Document 0098
 DOCN  M9580098
 TI    Construction of gag, pol, and env specific riboprobes for confirmation
       of HIV-1 specific polymerase chain reaction products.
 DT    9506
 AU    Dolan J; Desselberger U; Regional Virus Laboratory, East Birmingham
       Hospital, UK.
 SO    J Virol Methods. 1995 Jan;51(1):131-9. Unique Identifier : AIDSLINE
       MED/95247865
 AB    Conserved regions of the gag, pol, and env genes of HIV-1 pBH10 DNA (gag
       nucleotides(nt)1508-1652, pol nt 2811-3118, env nt 7792-7934; Ratner et
       al., 1985) were amplified by the polymerase chain reaction (PCR) using
       oligonucleotides complementary to the termini of these regions as
       primers. Primer areas of the amplified DNA were then removed by
       digestion with restriction endonucleases, and the internal fragments
       purified and cloned in both orientations into the 'riboprobe'
       transcription vector pGEM-5Z. Riboprobes made from these plasmids did
       detect the specific sequences of pBH10 DNA and of HIV-1 DNA amplified by
       PCR from clinical material. The riboprobes will be useful to confirm the
       specificity of PCR-amplified fragments of lymphocyte DNA obtained from
       infants of HIV-infected mothers and from high risk, but seronegative
       contacts of HIV-1 infected individuals.
 DE    Cloning, Molecular  DNA, Viral/GENETICS  Female  *Genes, env  *Genes,
       gag  *Genes, pol  Human  HIV Infections/DIAGNOSIS/VIROLOGY
       HIV-1/*GENETICS/ISOLATION & PURIF  Infant  Lymphocytes/VIROLOGY
       Molecular Probe Techniques  Nucleic Acid Hybridization  Polymerase Chain
       Reaction/*METHODS  Pregnancy  *RNA Probes  Support, Non-U.S. Gov't
       Virology/METHODS  CORRECTED AND REPUBLISHED ARTICLE  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).