Document 0099
 DOCN  M9580099
 TI    Evaluation of an agglutination HIV-1 + 2 antibody assay.
 DT    9506
 AU    Vercauteren G; Beelaert G; van der Groen G; Institute of Tropical
       Medicine, Department of Microbiology,; Antwerp, Belgium.
 SO    J Virol Methods. 1995 Jan;51(1):1-8. Unique Identifier : AIDSLINE
       MED/95247861
 AB    Studies have shown that an HIV (HIV-PA) agglutination assay (Serodia)
       for the detection of antibody to human immunodeficiency virus (HIV) can
       be as sensitive and as specific as enzyme-linked immunosorbent assay
       (ELISA). However, since this HIV assay was designed to detect antibody
       to the HIV-1 virus, a substantial number of HIV-2 positive sera are
       missed by this assay. Since the HIV-2 has now been found throughout the
       world this test is becoming less suitable. The new HIV-1 + 2 assay
       version (HIV-1 + 2 PA) was evaluated in 300 sera, which contained 50
       HIV-1, 40 HIV-2 and 10 HIV-1/HIV-2 antibody positive samples, and a
       sensitivity and specificity of 100% and 99%, respectively, was obtained.
       Whereas all HIV-2 positive sera were detected by the new HIV-1 + 2
       version, 26% (13/50) were missed by the old version of the agglutination
       test. It is concluded that the HIV-1 + 2 PA assay is a promising
       instrument free assay which can be used for screening purposes in areas
       where both HIV-1 and HIV-2 are present.
 DE    Agglutination Tests/*METHODS/STATISTICS & NUMER DATA  AIDS
       Serodiagnosis/*METHODS/STATISTICS & NUMER DATA  Comparative Study
       Enzyme-Linked Immunosorbent Assay/METHODS/STATISTICS & NUMER DATA
       Evaluation Studies  Human  HIV Antibodies/*BLOOD  HIV
       Seropositivity/DIAGNOSIS/IMMUNOLOGY  HIV-1/*IMMUNOLOGY
       HIV-2/*IMMUNOLOGY  Sensitivity and Specificity
       Virology/METHODS/STATISTICS & NUMER DATA  JOURNAL ARTICLE

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