Document 0101
 DOCN  M9580101
 TI    Stimulation of HIV expression by intracellular calcium pump inhibition.
 DT    9506
 AU    Papp B; Byrn RA; Hematology-Oncology Research Laboratory, Deaconess
       Hospital,; Harvard Medical School, Boston, Massachusetts 02215, USA.
 SO    J Biol Chem. 1995 Apr 28;270(17):10278-83. Unique Identifier : AIDSLINE
       MED/95247739
 AB    We have studied the role of intracellular calcium sequestration on human
       immunodeficiency virus (HIV) production by latently infected
       T-lymphocytic cells. Inhibition of the sarco-endoplasmic reticulum-type
       calcium transport ATPases by thapsigargin or cyclopiazonic acid induced
       activation of HIV production in the CEM-derived ACH-2 cells. An
       approximately 50% depletion of the thapsigargin-sensitive calcium pools
       as measured fluorimetrically of Indo-loaded cells fully activated virus
       production. Viral activation was manifest by increases in soluble viral
       core p24 production, increases in cellular immunofluorescent staining
       for viral antigens, and increased viral transcription as measured by HIV
       long terminal repeat-directed expression of the chloramphenicol
       acetyltransferase reporter gene. Virus induction could be blocked in a
       dose-dependent manner by the calcium channel blocker econazole. Virus
       production by the Jurkat-derived HIV-1-inducible J1.1 cells was not
       significantly stimulated by thapsigargin. These data indicate that
       intracellular calcium pool function is involved in the control of the
       transcription of proviral HIV in a cell type-specific manner within the
       T-lymphoid lineage and that ACH-2 cells represent a useful model for the
       study of calcium dependent activation of the transcription of proviral
       HIV.
 DE    Ca(2+)-Transporting ATPase/ANTAGONISTS & INHIB/*PHYSIOLOGY
       Calcium/*PHYSIOLOGY  Cell Line  Chloramphenicol
       Acetyltransferase/GENETICS  Fluorescent Antibody Technique  Human
       Hydroquinones/PHARMACOLOGY  HIV-1/*PHYSIOLOGY  Indoles/PHARMACOLOGY
       Kinetics  Support, Non-U.S. Gov't  Terpenes/PHARMACOLOGY  *Virus
       Replication/DRUG EFFECTS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).