Document 0110
 DOCN  M9580110
 TI    Impact of antigen-presenting cells on cytokine profiles of human Th
       clones established after stimulation with Mycobacterium tuberculosis
       antigens.
 DT    9506
 AU    Conradt P; Kaufmann SH; Department of Immunology, University of Ulm,
       Germany.
 SO    Infect Immun. 1995 May;63(5):2079-81. Unique Identifier : AIDSLINE
       MED/95247305
 AB    Human T cells reactive with mycobacterial antigens are generally
       considered to correlate with a Th1 cytokine profile. Our data show that,
       in addition, Th0 and Th2 clones develop in bulk culture with appropriate
       antigen-presenting cells before cloning. CD4+ blasts activated by
       mycobacterial antigens were cloned, and their mRNA patterns for the
       interleukins (IL) IL-2, IL-4, IL-5, IL-6, and IL-10 and gamma interferon
       were characterized by reverse-transcribed PCR. Nonadherent,
       nonrosetting, enriched peripheral blood mononuclear cells promoted
       development of Th0; after further depletion of monocytes and natural
       killer cells, Th2 clones were also found. Epstein-Barr virus-transformed
       B cells, with specificity for the stimulating antigen, increased the
       proportion of Th2 clones.
 DE    Antigen-Presenting Cells/*IMMUNOLOGY  Antigens, Bacterial/*IMMUNOLOGY
       Clone Cells  Cytokines/*BIOSYNTHESIS/GENETICS  CD4-Positive
       T-Lymphocytes/CYTOLOGY/IMMUNOLOGY  Human  Interferon Type
       II/ANALYSIS/GENETICS  Interleukins/ANALYSIS/GENETICS  Mycobacterium
       tuberculosis/*IMMUNOLOGY  RNA, Messenger/ANALYSIS  Support, Non-U.S.
       Gov't  T-Lymphocytes, Helper-Inducer/*IMMUNOLOGY  Th2 Cells/IMMUNOLOGY
       JOURNAL ARTICLE

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