Document 0122
 DOCN  M9580122
 TI    [Optimal conditions of gamma type irradiation for inactivating HIV in
       bone fragments. Consequences in biomechanical resistance of the bone
       tissue]
 DT    9506
 AU    Tosello A; Centre Regional de Transfusion Sanguine, Marseille.
 SO    Chirurgie. 1994-95;120(2):104-6. Unique Identifier : AIDSLINE
       MED/95246509
 AB    In order to determine bone-dependent penetration differences, we
       conducted a dosimetric study of gamma radiation using 2.5 mega rads on
       cancellous bone fragments at 4 degrees C and below 0 degrees with or
       without a surrounding solution. Variations in dose received inside and
       outside the femoral head were independent of radiation temperature but
       varied with the presence or absence of a surrounding solution. We then
       verified the gamma radiation effect on femoral heads infected with HIV:
       as free virus and in chronically infected cells. we evaluated infectious
       capacity after radiation by visualizing syncitia formation and by
       reverse transcriptase assay. The results for the controls were negative
       for free virus and positive for chronically infected cells. The virus
       was still after radiation. At the same time, we studied the effect of
       gamma radiation at different doses (0, 2.5, 5 mega rads) on
       biomechanical characteristics of bone, particularly crush resistance.
       Statistical analysis of the compression curves showed that resistance
       declined after high radiation at 5 mega rads, but the mean values
       obtained were within the range of values commonly accepted for human
       bone crush resistance.
 DE    Animal  Biomechanics  Bone and Bones/*RADIATION EFFECTS/VIROLOGY
       English Abstract  Femur Head/RADIATION EFFECTS/VIROLOGY  Gamma Rays
       Human  HIV/*RADIATION EFFECTS  HIV Infections/*RADIOTHERAPY  *Radiation
       Dosage  JOURNAL ARTICLE

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