Document 0145
 DOCN  M9580145
 TI    CD8+ T cells from a patient with colon carcinoma, specific for a mutant
       p21-Ras-derived peptide (Gly13-->Asp), are cytotoxic towards a carcinoma
       cell line harbouring the same mutation.
 DT    9506
 AU    Fossum B; Olsen AC; Thorsby E; Gaudernack G; Institute of
       Transplantation Immunology, National Hospital,; University of Oslo,
       Norway.
 SO    Cancer Immunol Immunother. 1995 Mar;40(3):165-72. Unique Identifier :
       AIDSLINE MED/95246048
 AB    Several T lymphocyte clones (TLC), specific for a p21-Ras-derived
       peptide expressing a Gly13-->Asp mutation and of the CD8+ subtype, were
       generated from peripheral blood of a colon carcinoma patient. The TLC
       exerted cytotoxicity against an interferon-gamma (IFN gamma)-pretreated
       colon carcinoma cell line, HCT116, which harbours the Gly13-->Asp
       mutation and shares both HLA-A2 and HLA-B12(44) with the patient. This
       cytotoxic effect could be blocked by a monoclonal antibody (mAb) against
       CD8 molecules, as well as with a mAb against HLA class I molecules and a
       polyclonal antiserum against HLA-B12, identifying B12(44) as the
       antigen-presenting molecule. In growth-inhibition experiments, the
       growth of both IFN gamma-pretreated and untreated target cells were
       strongly inhibited by the presence of the CD8+ TLC. Together these data
       indicate that human cancer cells harbouring a spontaneous ras mutation
       can process aberrant p21 Ras and express peptide/HLA-class-I complexes
       on their surface in sufficient density to be recognized by Ras-specific
       cytotoxic T lymphocytes.
 DE    Adenocarcinoma/GENETICS/*IMMUNOLOGY  Carcinogenicity Tests  Case Report
       Clone Cells  Colonic Neoplasms/GENETICS/*IMMUNOLOGY  Cytotoxicity Tests,
       Immunologic  *Cytotoxicity, Immunologic  CD8-Positive
       T-Lymphocytes/*IMMUNOLOGY  Female  Human  HLA-B Antigens/IMMUNOLOGY
       Middle Age  Point Mutation/*IMMUNOLOGY  Proto-Oncogene Protein
       p21(ras)/*GENETICS  Support, Non-U.S. Gov't  Tumor Cells, Cultured
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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