Document 0147
 DOCN  M9580147
 TI    Systemic antitumor effects of electrochemotherapy combined with
       histoincompatible cells secreting interleukin-2.
 DT    9506
 AU    Mir LM; Roth C; Orlowski S; Quintin-Colonna F; Fradelizi D; Belehradek J
       Jr; Kourilsky P; Laboratoire de Pharmacologie Moleculaire, URA 147 CNRS,
       Institut; Gustave-Roussy, Villejuif, France.
 SO    J Immunother Emphasis Tumor Immunol. 1995 Jan;17(1):30-8. Unique
       Identifier : AIDSLINE MED/95245502
 AB    Electrochemotherapy is an antitumor treatment that combines a cytotoxic
       drug with the local administration of electric pulses delivered at the
       tumor site. We previously found that in mice the cure rate of
       subcutaneous transplanted tumors treated by electrochemotherapy is
       increased by repeated systemic interleukin-2 (IL-2) injections.
       Moreover, histoincompatible cells engineered to secrete IL-2 allow the
       rejection of syngeneic tumor cells when both cells are inoculated
       together. In this study of preestablished tumors in mice we show that
       after electrochemotherapy, delayed peritumoral injections of
       histoincompatible IL-2-producing cells result in the cure of almost all
       the tumors. Moreover, this combined local treatment leads to cures of
       untreated, contralaterally transplanted tumors. This systemic antitumor
       immunity also resulted in complete protection of the cured mice against
       further inocula of the tumor cells. These results, which were obtained
       using allogeneic as well as xenogeneic IL-2-secreting cells, suggest
       that electrochemotherapy combined with such cellular immunotherapy might
       be a useful approach for the treatment of metastasizing cancers.
 DE    Animal  Bleomycins/*THERAPEUTIC USE  Carcinoma, Lewis Lung/*DRUG
       THERAPY/IMMUNOLOGY  Cells, Cultured  Combined Modality Therapy
       CD4-Positive T-Lymphocytes/IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/IMMUNOLOGY  CHO Cells  Edema/IMMUNOLOGY  *Electric
       Stimulation Therapy  Hamsters  Immunotherapy, Adoptive
       Interleukin-2/GENETICS/*THERAPEUTIC USE  Lymphocytes,
       Tumor-Infiltrating/IMMUNOLOGY  Mice  Mice, Inbred C57BL  Sarcoma,
       Experimental/*DRUG THERAPY/IMMUNOLOGY  Support, Non-U.S. Gov't
       T-Lymphocytes/*IMMUNOLOGY  *Transfection  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).