Document 0005
 DOCN  M9590005
 TI    Oral Candida albicans from patients infected with the human
       immunodeficiency virus and characterization of a genetically distinct
       subgroup of Candida albicans.
 DT    9509
 AU    McCullough M; Ross B; Reade PC; School of Dental Science, University of
       Melbourne.
 SO    Aust Dent J. 1995 Apr;40(2):91-7. Unique Identifier : AIDSLINE
       MED/95305798
 AB    Candida albicans has been shown to vary in its phenotypic expression
       with the progression of human immunodeficiency virus (HIV) infection.
       This study was designed to investigate whether in Category IV HIV
       infected patients (CDC, Atlanta, USA) these phenotypic changes were
       related to changes in the genetic strain of the organism. Isolates of C.
       albicans were obtained from 45 patients with HIV infection during the
       progression of their disease as determined by percentage T4 lymphocyte
       count. Isolates were strain differentiated using two methods. In 67 per
       cent of the patients a single strain of C. albicans, as determined by
       the DNA analysis, was isolated from each individual over the
       experimental period. The phenotypic expression of the genetically
       identical strains isolated from each patient varied considerably over
       the experimental period with the morphotype 754 being predominant. These
       results showed that the genotype of C. albicans isolated persisted in
       the majority of HIV infected individuals, but that the phenotypical
       expression of this strain changed. A finding in this study was that 18
       strains of C. albicans had DNA which did not hybridize to the probe
       used. These strains were analysed for the presence of two other C.
       albicans specific DNA segments using PCR. The probe 27A hybridizing
       strains yielded PCR products which differed from the non-hybridizing
       strains. Five of these genetically atypical C. albicans strains and 98
       of the C. albicans strains were then analysed for purported virulence
       factors.(ABSTRACT TRUNCATED AT 250 WORDS)
 DE    Acquired Immunodeficiency Syndrome/IMMUNOLOGY/*MICROBIOLOGY  Antifungal
       Agents/PHARMACOLOGY  AIDS-Related Opportunistic Infections/MICROBIOLOGY
       Candida albicans/DRUG EFFECTS/*GENETICS/ISOLATION & PURIF  Candidiasis,
       Oral/MICROBIOLOGY  Cell Adhesion/GENETICS  CD4 Lymphocyte Count  Drug
       Resistance, Microbial  DNA Probes  DNA, Fungal/ANALYSIS  Genotype  Human
       HIV Infections/IMMUNOLOGY/*MICROBIOLOGY  Mouth/*MICROBIOLOGY  Mouth
       Mucosa/CYTOLOGY  Peptide Peptidohydrolases/GENETICS/METABOLISM
       Phenotype  Polymerase Chain Reaction  Support, Non-U.S. Gov't
       Virulence/GENETICS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).