Document 0012
 DOCN  M9590012
 TI    Detection of bovine immunodeficiency virus in blood and milk-derived
       leukocytes by use of polymerase chain reaction.
 DT    9509
 AU    Nash JW; Hanson LA; St Cyr Coats K; Department of Biological Sciences,
       Mississippi State University,; MS 39762-5759, USA.
 SO    Am J Vet Res. 1995 Apr;56(4):445-9. Unique Identifier : AIDSLINE
       MED/95305359
 AB    Bovine immunodeficiency virus (BIV) is prevalent in beef and dairy
       cattle, yet the mode(s) of BIV transmission are undefined. Using
       polymerase chain reaction, which specifically targeted a 235-bp, highly
       conserved region of the BIV pol gene, BIV-infected leukocytes were
       detected in the blood and milk of BIV-seropositive cows. These data
       confirm the presence of BIV in milk and identify the potential for
       lactogenic transmission of this virus.
 DE    Animal  Base Sequence  Blood/*VIROLOGY  Cattle  *Cattle Diseases  Cells,
       Cultured  Comparative Study  Consensus Sequence  DNA Primers  DNA,
       Viral/ANALYSIS  Enzyme-Linked Immunosorbent Assay/METHODS/VETERINARY
       Female  Immunodeficiency Virus, Bovine/GENETICS/*ISOLATION & PURIF
       Lentivirus Infections/DIAGNOSIS/*VETERINARY  Leukocytes/*VIROLOGY  Lung
       Milk/*CYTOLOGY/*VIROLOGY  Molecular Sequence Data  Polymerase Chain
       Reaction/METHODS/*VETERINARY  Proviruses/GENETICS/ISOLATION & PURIF
       Sequence Homology, Nucleic Acid  Support, Non-U.S. Gov't  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).