Document 0045
 DOCN  M9590045
 TI    Versatile vectors to study recoding: conservation of rules between yeast
       and mammalian cells.
 DT    9509
 AU    Stahl G; Bidou L; Rousset JP; Cassan M; Institut de Genetique et
       Microbiologie, URA CNRS 1354,; Universite Paris-Sud, Orsay, France.
 SO    Nucleic Acids Res. 1995 May 11;23(9):1557-60. Unique Identifier :
       AIDSLINE MED/95303640
 AB    In many viruses and transposons, expression of some genes requires
       alternative reading of the genetic code, also called recoding. Such
       events depend on specific mRNA sequences and can lead to read through of
       an in-frame stop codon or to +1 or -1 frameshifting. Here, we addressed
       the issue of conservation of recoding rules between the yeast
       Saccharomyces cerevisiae and mammalian cells by establishing a versatile
       vector that can be used to study recoding in both species. We first
       assessed this vector by analysing the site of +1 frameshift of the Ty1
       transposon. Two sequences from higher organisms were then tested in both
       yeast and mammalian cells: the gag-pol junction of human
       immunodeficiency virus type 1 (HIV-1) (a site of -1 frameshift), and the
       stop codon region of the replicase cistron from the tobacco mosaic virus
       (a site of UAG read through). We show that both sequences direct a high
       level of recoding in yeast. Furthermore, different mutations of the
       target sequences have similar effects on recoding in yeast and in mouse
       cells. Most notably, a strong decrease of frameshifting was observed in
       the absence of the HIV-1 stem-loop stimulatory signal. Taken together,
       these data suggest that mechanisms of some recoding events are conserved
       between lower and higher eukaryotes, thus allowing the use of S.
       cerevisiae as a model system to study recoding on target sequences from
       higher organisms.
 DE    Animal  Base Sequence  Conserved Sequence  Frameshift Mutation  *Genetic
       Vectors  Human  HIV-1/*GENETICS  Mammals  Molecular Sequence Data  RNA,
       Messenger/*GENETICS  Saccharomyces cerevisiae  Sequence Alignment
       Support, Non-U.S. Gov't  Tobacco Mosaic Virus/*GENETICS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).