Document 0058
 DOCN  M9590058
 TI    Influence of exogenous interleukin-2 concentration on the isolation of
       human immunodeficiency virus.
 DT    9509
 AU    Zollner B; Feucht HH; Hamann A; Laufs R; Institut fur Medizinische
       Mikrobiologie und Immunologie,; Universitatsklinikum Eppendorf, Hamburg,
       Germany.
 SO    Microbiol Immunol. 1995;39(2):117-21. Unique Identifier : AIDSLINE
       MED/95303029
 AB    A significant increase (P = 0.015) in the HIV isolation rate from plasma
       samples was achieved by use of 10 U/ml exogenous interleukin-2 compared
       to 20 U/ml. The sensitivity rose from 0% to 29% in patients negative for
       p24 core antigen (P = 0.031) and from 71% to 86% in patients positive
       for p24 core antigen in plasma (P > 0.05). Titration of infectious HIV
       revealed that 10 U/ml interleukin-2 is the optimal concentration to
       isolate low numbers of infectious particles of HIV.
 DE    Acquired Immunodeficiency Syndrome/BLOOD/MICROBIOLOGY  Cells, Cultured
       DNA, Viral/ANALYSIS  Enzyme-Linked Immunosorbent Assay  Flow Cytometry
       Human  HIV Core Protein p24/ANALYSIS  HIV Infections/*BLOOD/MICROBIOLOGY
       HIV-1/GROWTH & DEVELOPMENT/GENETICS/*ISOLATION & PURIF
       Interleukin-2/*PHARMACOLOGY  Leukocytes, Mononuclear/DRUG
       EFFECTS/*MICROBIOLOGY  Microbiological Techniques  Polymerase Chain
       Reaction  Sensitivity and Specificity  Viremia/MICROBIOLOGY  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).