Document 0070
 DOCN  M9590070
 TI    [Induction of protective immune responses by a chimeric soluble protein
       from a recombinant BCG vector candidate vaccine to HIV-1]
 DT    9509
 AU    Honda M; Kitamura K; Okamoto Y; Watanabe K; Yoshizaki H; Fukushima Y;
       Naganawa S; Miyamoto G; Someya K; Yamada K; et al; National Institute of
       Health, Tokyo.
 SO    Rinsho Ketsueki. 1995 May;36(5):435-41. Unique Identifier : AIDSLINE
       MED/95302655
 AB    We have been isolated HIV strains from blood specimens of HIV infected
       individuals in Japan for these 6 years. The number of specimens tested
       reached approximately 1,700 that ninety percent of them were from
       hemophiliacs repeatedly injected blood products from the United States.
       More than 300 of field HIV were successfully isolated from the samples.
       The isolation rates has decreased to 30 percent in 1993 from 40 percent
       in 1992, suggesting that treatment with anti-HIV drugs such as AZT
       and/or ddI may be effective to HIV-infected individuals. Further, both
       of the viral and genomic sequences of HIV were classified to be clade B
       virus. The clinical isolates that expressed IHIGPGRAFY sequence at the
       center of the HIV-V3 domain were found to be neutralized by an
       anti-clade B-V3 monoclonal antibody, mu 5.5. By individual levels, when
       asymptomatic seropositives have progressed to disease-states,
       neutralization core motif of GPGR in approximately 6% of the viruses has
       changed to GPGG and hydrophilic amino acid changed to hydrophobic amino
       acid, correlating the loss of binding activity to PND-peptide of
       Japanese Consensus virus. Further, rapid progressors to HIV-induced
       diseases showed decreased activity of the binding antibody. By using the
       Japanese consensus sequence of HIV-1, we successfully constructed
       chimeric protein secretion vectors by selecting an appropriate insertion
       site of a carrier protein, and established the PND-peptide secretion
       system in BCG. The recombinant BCG inoculated guinea pigs were initially
       screened by delayed-type hypersensitivity (DTH) skin reactions to the
       PND peptide followed by passive transfer of the DTH by the systemic
       route.(ABSTRACT TRUNCATED AT 250 WORDS)
 DE    Animal  Antibody Formation  AIDS Vaccines/*IMMUNOLOGY  Base Sequence
       English Abstract  Guinea Pigs  Human  HIV-1/*IMMUNOLOGY  Mice  Molecular
       Sequence Data  Mycobacterium bovis/*IMMUNOLOGY  Neutralization Tests
       Recombinant Proteins/IMMUNOLOGY  Transfection  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).