Document 0074
 DOCN  M9590074
 TI    [An enzyme-linked immunosorbent assay determining anti-principal
       neutralizing determinant antibodies using synthetic peptides deduced
       from cDNA sequences of HIV-1 V3 loop domain]
 DT    9509
 AU    Ishimoto S; Fujimura Y; Yamanaka T; Shimoyama T; Nishida S; Matsuoka H;
       Nishikawa K; Yoshioka A; Narita N; Honda M; et al; Blood Transfusion
       Service, Nara Medical University.
 SO    Rinsho Ketsueki. 1995 Mar;36(3):193-9. Unique Identifier : AIDSLINE
       MED/95302624
 AB    The V3 domain, one of the hypervariable regions of gp 120 in HIV-1
       possesses principal type-specific neutralizing determinant (PND). The V3
       domain has the epitopes for class I major histocompatibility antigens of
       cytotoxic-T lymphocyte and helper-T-lymphocyte recognition sites, and
       also has major determinants for cell tropism towards macrophage,
       microglia, and human brain-derived fibroblast. Therefore, establishment
       for the assay of anti-PND antibodies in patients with HIV-1 infection
       appears to be important for the estimation on developing AIDS in these
       patients. We here describe a simple enzyme-linked immunosorbent assay
       (ELISA) for the measurement of anti-PND antibodies found in Japanese
       hemophiliacs. ELISA was performed using synthetic peptides, each 15
       amino acid residues deduced from cDNA sequences of seven HIV-1 V3 domain
       mutants, which include 5 North-American and European strains (IIIB, MN,
       RF, SC, and WMJ-2) and 2 African strains (Af1. Con and Af2, Con). The
       specific antibody binding to each peptide was determined after
       subtraction of the non-specific binding from the total. In 49 control
       sera from healthy individuals with HIV-1 antibody negative, the
       absorbance (M +/- 1SD) at 405 nm was -0.002 +/- 0.064. Then, the cut off
       value was determined to be M + 4SD. In 48 hemophiliac sera with HIV-1
       antibody negative, the absorbance was uniformly less than the cut off
       value. However, among 44 hemophiliac sera with HIV-1 antibody positive,
       the anti-PND antibodies were detected in the following frequencies; 2
       for IIIB, 20 for MN, 1 for RF, 1 for Af1. Con, and 5 for Af2.
       Con.(ABSTRACT TRUNCATED AT 250 WORDS)
 DE    Adult  Amino Acid Sequence  *Antigenic Determinants  AIDS Serodiagnosis
       English Abstract  Enzyme-Linked Immunosorbent Assay
       Hemophilia/IMMUNOLOGY  Human  HIV Antibodies/*ANALYSIS  HIV
       Antigens/*IMMUNOLOGY  HIV Envelope Protein gp120/*IMMUNOLOGY
       Immunoglobulin Variable Region/*IMMUNOLOGY  Middle Age  Molecular
       Sequence Data  Peptides/*IMMUNOLOGY  Support, Non-U.S. Gov't  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).