Document 0082
 DOCN  M9590082
 TI    T cell receptor distribution in rheumatoid synovial follicles.
 DT    9509
 AU    Travaglio-Encinoza A; Chaouni I; Dersimonian H; Jorgensen C;
       Simony-Lafontaine J; Romagne F; Sany J; Dupuy d'Angeac AD; Brenner MB;
       Reme T; Institut National de la Sante et de la Recherche Medicale;
       (INSERM), Unite 291, Montpellier, France.
 SO    J Rheumatol. 1995 Mar;22(3):394-9. Unique Identifier : AIDSLINE
       MED/95302336
 AB    OBJECTIVE. In rheumatoid arthritis (RA) joint erosion is accompanied by
       T cell infiltration of the synovial tissue. The resulting T cell
       receptor (TCR) repertoire is a combination of antigen driven shaping,
       nonspecific selection by endothelial cell-T cell interactions, and
       cytokine mediated chemoattraction. Considering the conflicting results
       of molecular biology studies of the TCR repertoire in RA, we attempted
       to obtain new data to clarify the situation through microscopic
       distribution analysis of TCR beta chain diversity in synovium follicular
       CD4/CD8 subsets. METHODS. We used dual color fluorescence confocal
       microscopy with CD4/CD8 monoclonal antibodies (Mab) and a panel of new
       anti-V beta Mab. The analysis focussed on lymphocyte rich, follicle-like
       areas of synovial tissue from 4 patients with RA. RESULTS. The
       expression of individual TCR beta families varied between areas within
       the T cell follicles, and between patients. Normal absolute levels of
       some beta chains can be completely skewed towards one subset, indicating
       that overall TCR evaluation is insufficient. CONCLUSION. Confocal
       microscopy analysis of localized TCR diversity in RA synovium offers
       novel insight into overall V beta gene usage, which appears to be the
       accumulation of several localized microexpansions.
 DE    Antibodies, Monoclonal  Arthritis,
       Rheumatoid/IMMUNOLOGY/*METABOLISM/PATHOLOGY  CD4-CD8 Ratio  Human
       Microscopy, Confocal  Receptors, Antigen, T-Cell, alpha-beta/*METABOLISM
       Support, Non-U.S. Gov't  Synovial
       Membrane/IMMUNOLOGY/*METABOLISM/PATHOLOGY  T-Lymphocyte
       Subsets/IMMUNOLOGY  Tissue Distribution  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).