Document 0085
 DOCN  M9590085
 TI    Intercellular adhesion molecule 3, a candidate human immunodeficiency
       virus type 1 co-receptor on lymphoid and monocytoid cells.
 DT    9509
 AU    Sommerfelt MA; Asjo B; Centre for Research in Virology, University of
       Bergen, Bergen; High Technology Centre, Norway.
 SO    J Gen Virol. 1995 Jun;76 ( Pt 6):1345-52. Unique Identifier : AIDSLINE
       MED/95302030
 AB    The CD4 molecule serves as the principal cell surface receptor common to
       both the human and simian immunodeficiency viruses (HIV-1, HIV-2 and
       SIV). Since binding to CD4 is not sufficient to permit virus entry, HIV
       'co-receptors' have been implicated in mediating the fusion of viral and
       cellular membranes necessary for completing the entry process. In order
       to identify candidate co-receptor molecules, a panel of monoclonal
       antibodies (MAbs) directed against adhesion molecules was tested for the
       ability of the MAbs to inhibit HIV-1-induced cell fusion (syncytium
       formation) and HIV-1 entry. Certain antibodies directed against CD18,
       CD11b and CD11c inhibited HIV-1-induced syncytium formation but not
       entry, in agreement with previous reports. Interestingly, certain
       antibodies to ICAM-3 (intercellular adhesion molecule 3) (CD50)
       significantly inhibited HIV-1-specific entry but not syncytium formation
       using human SupT1 cells. Only one antibody directed against ICAM-3
       significantly inhibited HIV-1-induced syncytium formation, entry and
       infectivity. Our results suggest that certain epitopes of ICAM-3 may be
       involved in mediating HIV-1-specific entry into lymphoid and monocytoid
       cells.
 DE    Antibodies, Monoclonal/PHARMACOLOGY  Antibody Specificity  Antigens,
       CD4/PHYSIOLOGY  Cell Adhesion Molecules/IMMUNOLOGY/*PHYSIOLOGY  Cell
       Line  Comparative Study  Giant Cells/IMMUNOLOGY  Hela Cells  Human
       HIV-1/*PHYSIOLOGY  Lymphocytes/IMMUNOLOGY/*VIROLOGY  Membrane Fusion
       Monocytes/IMMUNOLOGY/*VIROLOGY  Receptors, Virus/*PHYSIOLOGY  Reverse
       Transcriptase/ANALYSIS  Support, Non-U.S. Gov't  Vesicular
       Stomatitis-Indiana Virus/PHYSIOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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