Document 0087
 DOCN  M9590087
 TI    Human immunodeficiency virus type 1 infection of human CD4-transgenic
       rabbits.
 DT    9509
 AU    Dunn CS; Mehtali M; Houdebine LM; Gut JP; Kirn A; Aubertin AM; Unite
       INSERM U74, Faculte de Medecine, Universite Louis; Pasteur, Strasbourg,
       France.
 SO    J Gen Virol. 1995 Jun;76 ( Pt 6):1327-36. Unique Identifier : AIDSLINE
       MED/95302028
 AB    Investigations of human immunodeficiency virus (HIV) infection of man
       have benefited from the study of relevant animal models of the infection
       and disease. However, the ultimate models use primate species which are
       either endangered, not generally available, or expensive to maintain. A
       transgenic rabbit specifically and stably expressing human CD4 protein
       on T lymphocytes was assessed as a new laboratory animal model for HIV-1
       infection. In vitro studies demonstrate that lymphocytes derived from
       the transgenic rabbits are more susceptible to HIV-1IIIB infection than
       those from normal rabbits. In vivo infection of huCD4-transgenic rabbits
       using HIV-1IIIB-infected autologous lymphocytes was demonstrated by
       virus isolation, detection of HIV-1-specific DNA in peripheral blood
       lymphocytes and seroconversion to various HIV-1 proteins. Viral DNA was
       detected in the tissues of one rabbit sacrificed 7 weeks post-infection
       and virus was isolated from lymph node. Although these transgenic
       rabbits are less sensitive to HIV-1 infection than man, such a small and
       inexpensive animal model may be a useful tool.
 DE    Acquired Immunodeficiency Syndrome/*IMMUNOLOGY  Animal  Animals,
       Transgenic  Antigens, CD/*BIOSYNTHESIS  Antigens, CD4/*BIOSYNTHESIS
       Flow Cytometry  Genome, Viral  Human  HIV-1/ISOLATION &
       PURIF/*PHYSIOLOGY/PATHOGENICITY  Lymphocytes/*VIROLOGY  Organ
       Specificity  Polymerase Chain Reaction  Rabbits  Support, Non-U.S. Gov't
       Time Factors  JOURNAL ARTICLE

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