Document 0088
 DOCN  M9590088
 TI    Replication pattern of human immunodeficiency virus type 1 in mature
       Langerhans cells.
 DT    9509
 AU    Ludewig B; Holzmeister J; Gentile M; Gelderblom HR; Rokos K; Becker Y;
       Pauli G; Department of Virology, Robert Koch-Institut, Berlin, Germany.
 SO    J Gen Virol. 1995 Jun;76 ( Pt 6):1317-25. Unique Identifier : AIDSLINE
       MED/95302027
 AB    Langerhans cells (LC), the dendritic antigen presenting cells of the
       skin, mature into potent immunostimulatory cells during migration to
       regional lymph nodes, where they are identified as interdigitating cells
       (IDC). Since mature Langerhans cells (mLC) resemble IDC in phenotype and
       immunostimulatory capacity, we examined whether these cells were
       susceptible to infection with macrophagetropic and lymphotropic strains
       of human immunodeficiency virus type 1 (HIV-1). Highly purified cell
       preparations of mLC migrating from human epidermis expressed high
       amounts of major histocompatibility complex (MHC) class I and II
       antigens and of the accessory molecules CD40, CD80 and CD86, indicative
       of the phenotype of potent immunostimulatory cells. CD4 expression was
       upregulated on mLC during cultivation, independent of the presence of
       tumour necrosis factor alpha (TNF-alpha) and granulocyte-macrophage
       colony-stimulating factor (GM-CSF) in the culture medium. The
       macrophagetropic HIV-1 strain SF162 replicated to higher titres in mLC
       than the lymphotropic strain IIIB. Both strains induced syncytia, with
       SF162 showing a more rapid cytopathic effect. Addition of TNF-alpha
       enhanced virus production, due to better cell viability under TNF-alpha
       treatment, whereas GM-CSF did not significantly influence viability of
       cells and replication pattern of the virus. These findings suggest that
       in the infected individual IDC in lymph nodes may function as target
       cells for HIV-1.
 DE    Antibodies, Monoclonal  Antigens, CD/ANALYSIS  Cells, Cultured
       Cytokines/*PHARMACOLOGY  Human  HIV Core Protein p24/ANALYSIS
       HIV-1/DRUG EFFECTS/*PHYSIOLOGY/ULTRASTRUCTURE  Immunohistochemistry
       Kinetics  Langerhans Cells/*IMMUNOLOGY/ULTRASTRUCTURE/*VIROLOGY
       Microscopy, Electron  Support, Non-U.S. Gov't  *Virus Replication/DRUG
       EFFECTS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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