Document 0097
 DOCN  M9590097
 TI    Synthetic and biological macromolecules: protection of mouse embryos
       during cryopreservation by vitrification.
 DT    9509
 AU    Titterington JL; Robinson J; Killick SR; Hay DM; Department of Applied
       Biology, University of Hull, UK.
 SO    Hum Reprod. 1995 Mar;10(3):649-53. Unique Identifier : AIDSLINE
       MED/95301685
 AB    The ability of macromolecules to protect 8-cell mouse embryos during a
       vitrification protocol was assessed by the comparison of a synthetic
       macromolecule, polyvinylpyrrolidone (in the form of Percoll), and a
       biological macromolecule, human serum albumin (HSA). Vitrification
       solutions, which included glycerol (50% v/v), sucrose (0.75 mol/l) and
       either macromolecule Percoll (50% v/v) or HSA (1.125% w/v), were found
       to provide similar rates of survival. Both compounds resulted in a
       significant reduction in the incidence of disruption to the zonae
       pellucidae of cryopreserved embryos, to 4.8 +/- 1.3% and 10.3 +/- 1.2%
       respectively, when compared to the outcome when neither was present in
       the vitrification solution (20.4 +/- 2.5%; P < 0.01).
       Polyvinylpyrrolidone in the form of Percoll offers advantages over HSA
       in this work: it provides a lower rate of zona disruption and avoids the
       need for screening for pathogenic contaminants such as human
       immunodeficiency virus and hepatitis B and C.
 DE    Animal  Blastocyst/PHYSIOLOGY  Comparative Study  *Cryopreservation
       *Cryoprotective Agents  Embryo/*PHYSIOLOGY  Female  Glycerin  Human
       Male  Mice  Mice, Inbred CBA  *Povidone/*PHARMACOLOGY  Serum Albumin
       Silicon Dioxide/*PHARMACOLOGY  Solutions  Sucrose  Support, Non-U.S.
       Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).