Document 0098
 DOCN  M9590098
 TI    Distribution of leukocyte subtypes in the sheep ovary after laser
       drilling.
 DT    9509
 AU    Tozawa H; Brannstrom M; Petrucco O; Walker S; Chambers H; Pascoe V;
       Norman RJ; Department of Obstetrics and Gynaecology, University of
       Adelaide,; Woodville, Australia.
 SO    Hum Reprod. 1995 Mar;10(3):544-50. Unique Identifier : AIDSLINE
       MED/95301667
 AB    The distribution of leukocyte subtypes in the sheep ovary following
       laser drilling of the ovarian capsule was examined to understand a
       possible mechanism by which this treatment promotes ovulation in
       polycystic ovarian syndrome. Ovaries were removed from sheep at
       different time-points following laparoscopic laser drilling and
       immunohistochemical detection of leukocyte subtypes, using specific
       monoclonal antibodies; standard histological staining was performed.
       Migration of leukocytes into the laser-drilled site was observed as
       early as 6 h after laser drilling and the total number of leukocytes in
       the site was found to increase up to the 12th day after surgery. In the
       earlier period, polymorphonuclear leukocytes were the dominant leukocyte
       subtypes, while macrophages and lymphocytes were the major cellular
       components on the 12th day and later. These results show that the tissue
       changes in the ovary following laser drilling are consistent with a
       local inflammatory reaction. The prolonged appearance of numerous
       macrophages following the acute inflammatory phase could lead to the
       secretion of cytokines and other substances suggested to be important in
       promoting ovulation. These data indicate that part of the effectiveness
       of the laser drilling in polycystic ovarian syndrome may be attributable
       to the secretory products of these leukocytes.
 DE    Animal  Antibodies, Monoclonal  Antigens, CD/ANALYSIS  Antigens,
       CD45/ANALYSIS  CD4-Positive T-Lymphocytes/CYTOLOGY  CD8-Positive
       T-Lymphocytes/CYTOLOGY  Female  Histocompatibility Antigens Class
       II/ANALYSIS  Immunoenzyme Techniques  *Laser Surgery  Leukocyte Count
       Leukocytes/*CYTOLOGY/IMMUNOLOGY  Macrophages/CYTOLOGY
       Neutrophils/CYTOLOGY  Ovary/*CYTOLOGY/*SURGERY  Ovulation
       Induction/*METHODS  Sheep  T-Lymphocytes/CYTOLOGY/IMMUNOLOGY  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).