Document 0105
 DOCN  M9590105
 TI    Modulation of transcription factor NF kappa B activity by intracellular
       glutathione levels and by variations of the extracellular cysteine
       supply.
 DT    9509
 AU    Mihm S; Galter D; Droge W; Department of Immunochemistry, Deutsches
       Krebsforschungszentrum,; Heidelberg, Germany.
 SO    FASEB J. 1995 Feb;9(2):246-52. Unique Identifier : AIDSLINE MED/95301128
 AB    HIV-infected individuals and SIV-infected rhesus macaques have, on the
       average, decreased plasma cysteine and cystine concentrations and
       decreased intracellular glutathione levels. We now show that a depletion
       of intracellular glutathione in a human T cell line (Molt-4) inhibits
       the activation and nuclear translocation of the transcription factor NF
       kappa B, whereas incubation with increasing extracellular concentrations
       of cysteine inhibits the DNA-binding and transactivating activity of NF
       kappa B. Because inhibition of DNA-binding activity is associated with
       increasing intracellular glutathione disulfide levels and GSSG can be
       shown to inhibit the DNA-binding activity directly in cell-free systems,
       our studies suggest that GSSG is a physiologically relevant inhibitor in
       intact cells also. NF kappa B controls many immunologically important
       genes, so our studies suggest that the immune system may be sensitive
       not only against a cysteine and glutathione deficiency but also against
       an excess of cysteine.
 DE    Acetylcysteine/PHARMACOLOGY  Animal  Base Sequence  Binding Sites  Cell
       Line  Cell Nucleus/METABOLISM  Cysteine/*PHARMACOLOGY
       Dithiothreitol/PHARMACOLOGY  Dose-Response Relationship, Drug
       Glutathione/ANALOGS & DERIVATIVES/*METABOLISM  Human  HIV  HIV
       Infections/METABOLISM  Kinetics  Macaca mulatta  Molecular Sequence Data
       NF-kappa B/*METABOLISM  Oligodeoxyribonucleotides/METABOLISM  Simian
       Acquired Immunodeficiency Syndrome/METABOLISM  Sulfhydryl
       Compounds/METABOLISM  SIV  T-Lymphocytes  Trans-Activation
       (Genetics)/DRUG EFFECTS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).