Document 0107
 DOCN  M9590107
 TI    Rev-dependency of expression of human immunodeficiency virus type 1 gag
       and env genes.
 DT    9509
 AU    Sakai H; Furuta RA; Tokunaga K; Kawamura M; Hatanaka M; Adachi A;
       Institute for Virus Research, Kyoto University, Japan.
 SO    FEBS Lett. 1995 May 29;365(2-3):141-5. Unique Identifier : AIDSLINE
       MED/95300961
 AB    Structural gene expression of human immunodeficiency virus type 1
       (HIV-1) requires a viral regulatory protein, Rev transactivator. We
       investigated Rev-dependency of HIV-1 gene expression by various reporter
       systems. Expression of unspliced and single-spliced viral mRNAs was
       demonstrated to be differentially dependent on the Rev function. This
       difference of Rev-dependency was found not to be determined by
       cis-elements in gag, pol, and env coding sequences reported so far, and
       was lost when the reporter constructs containing minimum elements for
       Rev-responsiveness such as splice signals and rev responsive element
       were used for experiments. These findings indicated that the fundamental
       structure of HIV-1 mRNA was critical for the differential regulation of
       gene expression by Rev transactivator.
 DE    Animal  Cats  Cell Line  Cercopithecus aethiops  Chloramphenicol
       Acetyltransferase/ANALYSIS/BIOSYNTHESIS  Colonic Neoplasms  Comparative
       Study  *Gene Expression  Gene Expression Regulation, Viral  Gene
       Products, env/*BIOSYNTHESIS  Gene Products, gag/*BIOSYNTHESIS  Gene
       Products, rev/*METABOLISM  *Genes, env  *Genes, gag  Hamsters  Human
       HIV-1/GENETICS/*METABOLISM  Kidney  Mice  Recombinant
       Proteins/BIOSYNTHESIS  Reverse Transcriptase/ANALYSIS/BIOSYNTHESIS  RNA,
       Messenger/ANALYSIS/BIOSYNTHESIS  Support, Non-U.S. Gov't
       Trans-Activation (Genetics)  Transfection  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).