Document 0123
 DOCN  M9590123
 TI    High-performance liquid chromatographic assay for
       3'-amino-3'-deoxythymidine in plasma, with 9-fluorenyl methyl
       chloroformate as the derivatization agent.
 DT    9509
 AU    Nattouf H; Davrinche C; Sinet M; Farinotti R; Departement de Pharmacie
       Clinique, Faculte de Pharmacie,; Chatenay-Malabry, France.
 SO    J Chromatogr B Biomed Appl. 1995 Feb 17;664(2):365-71. Unique Identifier
       : AIDSLINE MED/95299665
 AB    We have developed a sensitive high-performance liquid chromatographic
       assay for the determination of the zidovudine metabolite
       3'-amino-3'-deoxythimidine (AMT) using fluorescence and sensitivity in
       the picomolar range. Plasma was diluted with 0.05 M sodium phosphate
       buffer pH 7.2 and subsequently prepared for analysis using solid-phase
       extraction. AMT was derivatized with 9-fluorenyl methylchloroformate and
       chromatographed using a reversed-phase system. The mobile phase
       consisted of acetonitrile-0.01 M potassium phosphate buffer (pH 7)
       (32.68, v/v). The fluorescence of the column effluent was monitored at
       262 nm (excitation) and 306 nm (emission). Good resolution of AMT from
       endogenous plasma components was obtained. Within- and between-day
       variability was less than 10%. The limit of quantitation was 0.9
       microgram/l. The assay was successfully applied to the determination of
       AMT in human plasma and in plasma of mice treated with zidovudine.
 DE    Animal  Calibration  Chromatography, High Pressure Liquid
       Dideoxynucleosides/*BLOOD  Fluorenes  Human  HIV Infections/BLOOD  Mice
       Mice, Inbred DBA  Quality Control  Spectrometry, Fluorescence
       Zidovudine/PHARMACOKINETICS  CLINICAL TRIAL  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).